Ninditya Vika Ichsania, Purwati Endah, Utami Ajeng Tyas, Marwaningtyaz Aprillyani Sofa, Fairuz Nadia Khairunnisa, Widayanti Rini, Hamid Penny Humaidah
Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia.
Study Program, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia.
Vet World. 2020 Jul;13(7):1423-1429. doi: 10.14202/vetworld.2020.1423-1429. Epub 2020 Jul 24.
is the vector of dengue fever, dengue hemorrhagic fever, chikungunya, and, most recently, Zika. Dengue fever is one of Indonesia's endemic diseases. The principal tool for preventing dengue is controlling by chemical insecticides since vaccine against dengue is still under research. However, developed resistance to various chemical insecticides worldwide. Therefore, research on alternate compounds as mosquito insecticides is urgently needed. This study demonstrated the efficacy of extract as larvicidal, ovicidal, adulticidal, repellency, and oviposition deterrent activity against .
was obtained from Temanggung, Indonesia, while the eggs of were collected from Yogyakarta, Indonesia, and were hatched in Laboratory of Parasitology, Faculty of Veterinary Medicine, Universitas Gadjah Mada. Larvicidal activity was evaluated according to the WHO protocol; adulticidal activity was performed using the Centers for Disease Control protocol. Oviposition activity was evaluated using ovitraps added with extract, complete protection time in the repellent assay was defined as the number of minutes elapsed between compound application and the landing of the first mosquito.
A test of the larvicidal activity of extract returned an LC of 65.8 ppm (r=0.9014) in 1 h and 18.6 ppm (r=0.575) in 24 h. was effective as an adulticidal, demonstrating LC values of 11.35 mg (r=0.875) in 90 min, 9.63 mg (r=0.924) in 105 min, and 6.46 mg (r=0.925) in 120 min. at a concentration of 1000 ppm was able to reach 96% of oviposition deterrent effect. The ovicidal assay, a concentration of 1000 ppm resulted in 82.67% of eggs remaining unhatched. An extract concentration of 80 mg/ml achieved 63.3±3.5% biting repellency in adults.
This study gives a clear indication that extract acts on at various developmental stages and is a potential alternative bioinsecticide for controlling this disease vector.
是登革热、登革出血热、基孔肯雅热以及最近的寨卡病毒的传播媒介。登革热是印度尼西亚的地方病之一。预防登革热的主要手段是使用化学杀虫剂控制，因为针对登革热的疫苗仍在研究中。然而，在全球范围内已对各种化学杀虫剂产生了抗性。因此，迫切需要研究替代化合物作为蚊虫杀虫剂。本研究证明了提取物对具有杀幼虫、杀卵、杀成虫、驱避和产卵抑制活性。
取自印度尼西亚坦戎贡，而的卵则从印度尼西亚日惹收集，并在加查马达大学兽医学院寄生虫学实验室孵化。杀幼虫活性根据世界卫生组织的方案进行评估；杀成虫活性使用疾病控制中心的方案进行。产卵活性通过添加提取物的诱蚊产卵器进行评估，驱避试验中的完全保护时间定义为从化合物施用至第一只蚊子着陆之间经过的分钟数。
提取物的杀幼虫活性测试在1小时内的LC50为65.8 ppm（r = 0.9014），在24小时内为18.6 ppm（r = 0.575）。作为杀成虫剂有效，在90分钟时的LC50值为11.35毫克（r = 0.875），在105分钟时为9.63毫克（r = 0.924），在120分钟时为6.46毫克（r = 0.925）。浓度为1000 ppm时能够达到96%的产卵抑制效果。在杀卵试验中，浓度为1000 ppm时导致82.67%的卵未孵化。提取物浓度为80毫克/毫升时，成虫的叮咬驱避率达到63.3±3.5%。
本研究清楚地表明提取物在的各个发育阶段都有作用，是控制这种病媒的一种潜在替代生物杀虫剂。
