Predoi Daniela, Iconaru Simona Liliana, Predoi Mihai Valentin
National Institute of Materials Physics, Atomistilor Street, No. 405A, P.O. Box MG 07, 077125 Magurele, Romania.
University Politehnica of Bucharest, BN 002, 313 Splaiul Independentei, Sector 6, 060042 Bucharest, Romania.
Polymers (Basel). 2019 May 15;11(5):886. doi: 10.3390/polym11050886.
Dextran-coated zinc-doped hydroxyapatite (ZnHApD) was synthesized by an adapted sol-gel method. The stability of ZnHApD nanoparticles in an aqueous solution was analyzed using ultrasonic measurements. The analysis of the evolution in time of the attenuation for each of the frequencies was performed. The X-ray diffraction (XRD) investigations exhibited that no impurity was found. The morphology, size and size distribution of the ZnHApD sample was investigated by transmission electron microscopy (TEM) and scanning electron microscopy (SEM). The TEM and SEM results showed that the ZnHApD particles have an ellipsoidal shape and a narrow distribution of sizes. The cell growth and toxicity of HEK-293 cells were investigated on the ZnHApD solution for four different concentrations and analyzed after 24 and 48 h. The ZnHApD solution presented a non-toxic activity against HEK-293 cells for all analyzed concentrations. The antibacterial assay revealed that all the tested microorganisms were inhibited by the ZnHApD dispersion after 24 and 48 h of incubation. It was observed that the effect of the ZnHApD solution on bacteria growth depended on the bacterial strain. The ATCC 33277 bacterial strain was the most sensitive, as a growth inhibition in the presence of 0.075 μg/mL ZnHApD in the culture medium was observed.
采用改进的溶胶 - 凝胶法合成了葡聚糖包覆的锌掺杂羟基磷灰石（ZnHApD）。利用超声测量分析了ZnHApD纳米颗粒在水溶液中的稳定性。对每个频率下衰减随时间的演变进行了分析。X射线衍射（XRD）研究表明未发现杂质。通过透射电子显微镜（TEM）和扫描电子显微镜（SEM）研究了ZnHApD样品的形态、尺寸和尺寸分布。TEM和SEM结果表明，ZnHApD颗粒呈椭圆形且尺寸分布狭窄。在四种不同浓度的ZnHApD溶液上研究了HEK - 293细胞的生长和毒性，并在24小时和48小时后进行分析。对于所有分析浓度，ZnHApD溶液对HEK - 293细胞均呈现无毒活性。抗菌试验表明，在孵育24小时和48小时后，所有测试微生物均被ZnHApD分散液抑制。观察到ZnHApD溶液对细菌生长的影响取决于细菌菌株。ATCC 33277菌株最为敏感，在培养基中存在0.075μg/mL ZnHApD时观察到生长抑制。
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