Predoi Daniela, Iconaru Simona Liliana, Predoi Mihai Valentin, Stan George E, Buton Nicolas
National Institute of Materials Physics, 405A Atomistilor Street, P.O. Box MG7, 077125 Magurele, Romania.
University Politehnica of Bucharest, BN 002, 313 Splaiul Independentei, Sector 6, 10023 Bucharest, Romania.
Nanomaterials (Basel). 2019 Sep 11;9(9):1295. doi: 10.3390/nano9091295.
Obtaining nanoscale materials has allowed for the miniaturization of components, which has led to the possibility of achieving more efficient devices with faster functions and much lower costs. While hydroxyapatite [HAp, Ca(PO)(OH)] is considered the most widely used material for medical applications in orthopedics, dentistry, and general surgery, the magnesium (Mg) is viewed as a promising biodegradable and biocompatible implant material. Furthermore, Mg is regarded as a strong candidate for developing medical implants due to its biocompatibility and antimicrobial properties against gram-positive and gram-negative bacteria. For this study, magnesium-doped hydroxyapatite (CaMg (PO) (OH), x = 0.1), 10MgHAp, suspensions were successfully obtained by an adapted and simple chemical co-precipitation method. The information regarding the stability of the nanosized 10MgHAp particles suspension obtained by ζ-potential analysis were confirmed for the first time by a non-destructive ultrasound-based technique. Structural and morphological studies of synthesized 10MgHAp were conducted by X-ray diffraction (XRD), Fourier-transform infrared (FTIR) spectroscopy in attenuated total reflectance (ATR) mode and scanning electron microscopy (SEM). The XRD analysis of the 10MgHAp samples confirmed that a single crystalline phase associated to HAp with an average grain size about 93.3 nm was obtained. The FTIR-ATR spectra revealed that the 10MgHAp sample presented broader IR bands with less visible peaks when compared to a well-crystallized pure HAp. The SEM results evidenced uniform MgHAp nanoparticles with spherical shape. The antimicrobial activity of the 10MgHAp suspension against gram-positive strains ( ATCC 25923, ATCC 29212), gram-negative strains ( ATCC 25922, ATCC 27853), as well as a fungal strain ( ATCC 90029) were evaluated.
获取纳米级材料使得部件能够小型化，这进而带来了实现功能更快、成本更低且效率更高的器件的可能性。虽然羟基磷灰石[HAp，Ca(PO)(OH)]被认为是在骨科、牙科和普通外科医学应用中使用最广泛的材料，但镁（Mg）被视为一种有前景的可生物降解且生物相容的植入材料。此外，由于镁的生物相容性以及对革兰氏阳性和革兰氏阴性细菌的抗菌特性，它被视为开发医用植入物的有力候选材料。在本研究中，通过一种经过改进的简单化学共沉淀法成功获得了镁掺杂的羟基磷灰石（CaMg(PO)(OH)，x = 0.1），即10MgHAp悬浮液。通过基于无损超声的技术首次证实了通过ζ电位分析获得的纳米级10MgHAp颗粒悬浮液的稳定性信息。通过X射线衍射（XRD）、衰减全反射（ATR）模式的傅里叶变换红外（FTIR）光谱以及扫描电子显微镜（SEM）对合成的10MgHAp进行了结构和形态学研究。对10MgHAp样品的XRD分析证实获得了与HAp相关的单相晶体，平均晶粒尺寸约为93.3 nm。FTIR-ATR光谱显示，与结晶良好的纯HAp相比，10MgHAp样品的红外波段更宽，峰不太明显。SEM结果证明了具有球形形状的均匀MgHAp纳米颗粒。评估了10MgHAp悬浮液对革兰氏阳性菌株（ATCC 25923、ATCC 29212）、革兰氏阴性菌株（ATCC 25922、ATCC 27853）以及一种真菌菌株（ATCC 90029）的抗菌活性。
