Jahanian E, Mahdavi A H, Asgary S, Jahanian R, Tajadini M H
Department of Animal Sciences, College of Agriculture, Isfahan University of Technology, Isfahan 84156-83111, Iran.
Department of Animal Sciences, College of Agriculture, Isfahan University of Technology, Isfahan 84156-83111, Iran.
Prev Vet Med. 2019 Jul 1;168:9-18. doi: 10.1016/j.prevetmed.2019.04.005. Epub 2019 Apr 15.
The present study was conducted to investigate the effects of dietary supplementation of mannanoligosaccharides (MOS) on expression of hepatic immunological genes and immune responses in aflatoxin-contaminated broiler chicks. A total of 336 seven-day-old Ross broiler chicks were randomly allotted to 7 experimental treatments with 4 replicates and 12 birds per replicate. Experimental treatments consisted of 2 aflatoxin levels (0.5 and 2 ppm) and 3 supplemental MOS levels (0, 1 and 2 g/kg) as a 2 × 3 factorial arrangement in comparison with a control group (unchallenged group). The chicks were challenged with a mix of aflatoxins during 7-28 d of age. Results showed that aflatoxin challenge resulted in the lower antibody titers against infectious bronchitis (IBV) and bursal (IBD) diseases viruses. In addition, aflatoxin-contaminated birds had a lower (P < 0.0001) lymphocyte percentage and a decline in (P < 0.01) interleukin-2 (IL-2) mRNA abundance. Likewise, heterophil proportion, heterophil to lymphocyte ratio and gene expressions of hepatic interleukin-6 (IL-6) and C reactive protein (CRP) were raised (P < 0.001) by increasing dietary aflatoxin level. Dietary inclusion of MOS increased (P < 0.05) antibody titers against IBV, IBD and Newcastle disease virus. Lymphocyte proportion and hepatic IL-2 gene expression were greater (P < 0.0001) in MOS-supplemented birds. Furthermore, supplemental MOS decreased hepatic IL-6 and CRP abundances. Additionally, inclusion of 2 g/kg MOS resulted in the upregulation (P < 0.01) of hepatic IL-2 gene expression in birds contaminated with 0.5 ppm aflatoxin. The present results indicate that supplemental MOS could improve cellular immunity via the upregulation of hepatic IL-2 gene expression in birds challenged with aflatoxins.
本研究旨在探讨日粮中添加甘露寡糖（MOS）对黄曲霉毒素污染的肉仔鸡肝脏免疫基因表达和免疫反应的影响。总共336只7日龄罗斯肉仔鸡被随机分配到7种实验处理组，每组4个重复，每个重复12只鸡。实验处理包括2种黄曲霉毒素水平（0.5和2 ppm）和3种MOS添加水平（0、1和2 g/kg），采用2×3析因设计，与对照组（未受挑战组）进行比较。在7至28日龄期间，用黄曲霉毒素混合物对雏鸡进行攻毒。结果表明，黄曲霉毒素攻毒导致针对传染性支气管炎（IBV）和法氏囊（IBD）疾病病毒的抗体滴度降低。此外，受黄曲霉毒素污染的鸡淋巴细胞百分比更低（P < 0.0001），白细胞介素-2（IL-2）mRNA丰度下降（P < 0.01）。同样，随着日粮黄曲霉毒素水平的增加，异嗜性粒细胞比例、异嗜性粒细胞与淋巴细胞比例以及肝脏白细胞介素-6（IL-6）和C反应蛋白（CRP）的基因表达升高（P < 0.001）。日粮中添加MOS可提高（P < 0.05）针对IBV、IBD和新城疫病毒的抗体滴度。添加MOS的鸡淋巴细胞比例和肝脏IL-2基因表达更高（P < 0.0001）。此外，添加MOS可降低肝脏IL-6和CRP丰度。此外，添加2 g/kg MOS可使受0.5 ppm黄曲霉毒素污染的鸡肝脏IL-2基因表达上调（P < 0.01）。目前的结果表明，添加MOS可通过上调受黄曲霉毒素攻击的鸡肝脏IL-2基因表达来改善细胞免疫。
