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几何结构、干燥和合成方法对氨基化壳聚糖包覆磁性纳米粒子用于 HSA 有效固定化的影响。

Effect of Geometrical Structure, Drying, and Synthetic Method on Aminated Chitosan-Coated Magnetic Nanoparticles Utility for HSA Effective Immobilization.

机构信息

Faculty of Chemistry, Nicolaus Copernicus University in Torun, Gagarina 7, 87-100 Torun, Poland.

Karlsruhe Inst Technol, Inst Nanotechnol INT, Hermann von Helmholtz Pl 1, D-76344 Eggenstein-Leopoldshafen, Germany.

出版信息

Molecules. 2019 May 18;24(10):1925. doi: 10.3390/molecules24101925.

Abstract

Human serum albumin (HSA) is one of the most frequently immobilized proteins on the surface of carriers, including magnetic nanoparticles. This is because the drug-HSA interaction study is one of the basic pharmacokinetic parameters determined for drugs. In spite of many works describing the immobilization of HSA and the binding of active substances, research describing the influence of the used support on the effectiveness of immobilization is missing. There are also no reports about the effect of the support drying method on the effectiveness of protein immobilization. This paper examines the effect of both the method of functionalizing the polymer coating covering magnetic nanoparticles (MNPs), and the drying methods for the immobilization of HSA. Albumin was immobilized on three types of aminated chitosan-coated nanoparticles with a different content of amino groups long distanced from the surface FeO-CS-Et(NH). The obtained results showed that both the synthesis method and the method of drying nanoparticles have a large impact on the effectiveness of immobilization. Due to the fact that the results obtained for FeO-CS-Et(NH) significantly differ from those obtained for the others, the influence of the geometry of the shell structure on the ability to bind HSA was also explained by molecular dynamics.

摘要

人血清白蛋白(HSA)是载体表面最常固定的蛋白质之一,包括磁性纳米粒子。这是因为药物-HSA 相互作用研究是确定药物的基本药代动力学参数之一。尽管有许多描述 HSA 固定化和活性物质结合的工作,但缺乏描述所使用的载体对固定化效果的影响的研究。也没有关于载体干燥方法对蛋白质固定化效果的影响的报道。本文研究了功能化聚合物涂层覆盖磁性纳米粒子(MNPs)的方法以及 HSA 固定化的干燥方法对其的影响。将白蛋白固定在三种具有不同氨基含量的氨化壳聚糖包覆的纳米粒子上,这些氨基与表面的 FeO-CS-Et(NH) 距离较远。所得结果表明,合成方法和纳米粒子干燥方法对固定化效果都有很大的影响。由于 FeO-CS-Et(NH) 的结果与其他结果明显不同,还通过分子动力学解释了壳层结构的几何形状对结合 HSA 能力的影响。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5dfa/6572529/c032b5312e48/molecules-24-01925-g001.jpg

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