Department of Animal Science, South Dakota State University, Brookings, SD.
Canadian Bio-Systems Inc., Calgary, AB, Canada.
J Anim Sci. 2019 Jul 2;97(7):2979-2988. doi: 10.1093/jas/skz178.
A study was conducted to determine effects of supplementing multi-enzyme on apparent ileal digestibility (AID) of energy and AA; and apparent total tract digestibility (ATTD) of energy for pigs fed low-energy and AA diets. Eight ileal-cannulated barrows (initial BW: 38.7 ± 2.75 kg) were fed four diets in a replicated 4 × 4 Latin square design to give 8 replicates per diet. Diets were positive control (PC) diet, negative control (NC) diet without or with multi-enzyme at 0.5 or 1.0 g/kg. The PC diet was formulated to meet or exceed NRC (2012) nutrient recommendations for grower pigs (25 to 50 kg), except for Ca and digestible P, which were lower than NRC (2012) recommendations by 0.13 and 0.17 percentage points, respectively, due to phytase supplementation at 1,000 FTU/kg. The NC diet was formulated to be lower in NE by 75 kcal/kg and standardized ileal digestible AA content by a mean of 3%. These reductions were achieved by partial replacement of corn and soybean meal (SBM) and complete replacement of soybean oil and monocalcium phosphate in PC diet with 25% corn distillers dried grains with solubles (DDGS) and 3.6% soybean hulls. Multi-enzyme at 1.0 g/kg supplied 1,900 U of xylanase, 300 U of β-glucanase, 1,300 U of cellulase, 11,500 U of amylase, 120 U of mannanase, 850 U of pectinase, 6,000 U of protease, and 700 U of invertase per kilogram of diet. The AID of GE, N, most AA, most component sugars of nonstarch polysaccharides (NSP) and P; ATTD of GE for PC diet was greater (P < 0.05) than those for NC diets. An increase in dietary level of multi-enzyme from 0 to 1.0 g/kg resulted in a linear increase (P < 0.05) in AID of Ile by 4.3%, and tended to linearly increase (P < 0.10) AID of Leu, Met, Phe, and Val by a mean of 3.4%. Increasing dietary multi-enzyme from 0 to 1.0 g/kg linearly increased (P < 0.05) AID of total NSP and P by 53.7% and 19.2%, respectively; ATTD of GE by 8.4% and DE and NE values by 8.8% and 8.2%, respectively; tended to linearly increase (P < 0.10) AID of GE by 8.1%. The NE values for NC diet with multi-enzyme at 1.0 g/kg tended to be greater (P < 0.10) than that for PC diet (2,337 vs. 2,222 kcal/kg of DM). In conclusion, multi-enzyme supplementation improved energy and nutrient digestibilities of a corn-SBM-corn DDGS-based diet, implying that the multi-enzyme fed in the current study can be used to enhance energy and nutrient utilization of low-energy AA diets for grower pigs.
进行了一项研究,以确定在低能量和氨基酸饮食中添加多酶对猪的表观回肠消化率(AID)的能量和 AA 以及表观全肠道消化率(ATTD)的能量的影响。将 8 头回肠结扎的阉公猪(初始体重:38.7 ± 2.75kg)饲喂四种日粮,在重复 4×4 拉丁方设计中进行,每种日粮有 8 个重复。日粮分别为阳性对照(PC)日粮、无或有 0.5 或 1.0g/kg 多酶的阴性对照(NC)日粮。PC 日粮的配方旨在满足或超过生长猪(25-50kg)的 NRC(2012)营养推荐值,除了钙和可消化磷,由于添加了 1000FTU/kg 的植酸酶,分别比 NRC(2012)推荐值低 0.13 和 0.17 个百分点。NC 日粮的 NE 降低了 75kcal/kg,标准化回肠可消化 AA 含量降低了 3%。这些减少是通过部分替代玉米和大豆粉(SBM)以及完全替代大豆油和磷酸氢钙来实现的,在 PC 日粮中用 25%玉米干酒糟及其可溶性物(DDGS)和 3.6%大豆皮来实现。1.0g/kg 的多酶提供了 1900U 的木聚糖酶、300U 的β-葡聚糖酶、1300U 的纤维素酶、11500U 的淀粉酶、120U 的甘露聚糖酶、850U 的果胶酶、6000U 的蛋白酶和 700U 的转化酶/kg 日粮。PC 日粮的 GE、N、大多数 AA、非淀粉多糖(NSP)和 P 的 AID;GE 的 ATTD 大于(P<0.05)NC 日粮。多酶的日粮水平从 0 增加到 1.0g/kg,导致 Ile 的 AID 线性增加(P<0.05),增加了 4.3%,并且趋于线性增加(P<0.10)Leu、Met、Phe 和 Val 的 AID,平均增加了 3.4%。多酶的日粮水平从 0 增加到 1.0g/kg,导致总 NSP 和 P 的 AID 分别线性增加(P<0.05)53.7%和 19.2%;GE 的 ATTD 增加了 8.4%,DE 和 NE 值分别增加了 8.8%和 8.2%;GE 的 AID 趋于线性增加(P<0.10)8.1%。添加 1.0g/kg 多酶的 NC 日粮的 NE 值趋于大于(P<0.10)PC 日粮(2337 比 2222kcal/kg DM)。总之,多酶的添加提高了基于玉米-SBM-玉米 DDGS 的日粮的能量和营养素消化率,这表明本研究中添加的多酶可以用于提高生长猪低能量 AA 日粮的能量和营养素利用。