Minami Masaaki, Takase Hiroshi, Taira Masayo, Makino Toshiaki
Department of Bacteriology, Graduate School of Medical Sciences, Nagoya City University, 1 Kawasumi, Mizuho-ku, Nagoya 467-8601, Japan.
Core Laboratory, Graduate School of Medical Sciences, Nagoya City University, 1 Kawasumi, Mizuho-ku, Nagoya 467-8601, Japan.
Medicines (Basel). 2019 May 20;6(2):58. doi: 10.3390/medicines6020058.
: Hainosan (Painongsan) is a traditional Japanese and Chinese medicine that is used to treat several purulent diseases, including gingivitis and periodontitis. This formulation contains three crude drug components: The dried immature fruit of (Aurantii Fructus Immaturus), the dried root of (Paeoniae Radix), and the dried root of (Platycodi Radix). Here we evaluated the in vitro antibacterial effects of hainosan extract (HNS) and extracts of its three components against , one of the pathogenic bacteria that causes periodontitis. The antibacterial activities of HNS and its components were examined by counting the number of colony-forming units (CFUs) and through transmission electron microscopy. We found that HNS had direct antibacterial activity against three isolates (JCM12257, JCM8525, and JCM19600), with HNS-treated cells being significantly smaller than those of untreated bacteria. Extracts of Platycodi Radix and Paeoniae Radix significantly suppressed the growth of in a dose-dependent manner, with Platycodi Radix extract having the greatest antibacterial effect. In addition, that were treated with Platycodi Radix extract were significantly larger than those treated with Aurantii Fructus Immaturus or Paeoniae Radix extracts. Further analysis showed that platycodin D, which is one of the ingredients of Platycodi Radix, reduced bacterial growth. Platycodi Radix is the active component in Hainosan and may represent a useful agent for the treatment of -induced gingivitis and periodontitis.
败毒散（排脓散）是一种传统的日汉药，用于治疗多种化脓性疾病，包括牙龈炎和牙周炎。该配方包含三种生药成分：枳实（酸橙未成熟果实干燥品）、芍药（芍药根干燥品）和桔梗（桔梗根干燥品）。在此，我们评估了败毒散提取物（HNS）及其三种成分的提取物对牙龈卟啉单胞菌（一种导致牙周炎的病原菌）的体外抗菌作用。通过计算菌落形成单位（CFU）数量并借助透射电子显微镜检查了HNS及其成分的抗菌活性。我们发现HNS对三种牙龈卟啉单胞菌分离株（JCM12257、JCM8525和JCM19600）具有直接抗菌活性，经HNS处理的细胞明显小于未处理细菌的细胞。桔梗提取物和芍药提取物以剂量依赖方式显著抑制牙龈卟啉单胞菌的生长，其中桔梗提取物的抗菌效果最佳。此外，经桔梗提取物处理的细胞明显大于经枳实或芍药提取物处理的细胞。进一步分析表明，桔梗中的成分之一桔梗皂苷D可减少细菌生长。桔梗是败毒散中的活性成分，可能是治疗牙龈卟啉单胞菌引起的牙龈炎和牙周炎的有效药物。
