College of Animal Science and Technology, Shandong Agricultural University, Tai'an 271000, China.
Biomed Res Int. 2019 Apr 16;2019:8159567. doi: 10.1155/2019/8159567. eCollection 2019.
This study aimed to investigate the prevalence and antimicrobial resistance of spp. isolated from large-scale breeder farms in Shandong Province, China. A total of 63 isolates (63/409, 15.4%) were identified from 409 samples collected from five large-scale breeder farms in Shandong Province. These isolates were assayed for serotype, antimicrobial susceptibility, prevalence of class 1 integrons, quinolone resistance genes, and -lactamase genes and subtyped by multilocus sequence typing (MLST). Among these isolates, . Enteritidis (100%) was the predominant serovar, and high antimicrobial resistance rates to nalidixic acid (100.0%), streptomycin (100.0%), ampicillin (98.4%), and erythromycin (93.7%) were observed. All of the isolates carried blaTEM. MLST results showed that only one sequence type (ST11) was identified. Our findings indicated that was generally prevalent not only on broiler farms but also on breeder farms.
本研究旨在调查中国山东省大型养殖场中分离的 spp. 的流行情况和抗药性。从山东省五个大型养殖场的 409 个样本中采集了 63 株分离株(63/409,15.4%)。对这些分离株进行血清型鉴定、抗菌药物敏感性试验、1 类整合子流行率、喹诺酮类耐药基因和β-内酰胺酶基因检测,并进行多位点序列分型(MLST)。在这些分离株中,. Enteritidis(100%)是主要血清型,对萘啶酸(100.0%)、链霉素(100.0%)、氨苄西林(98.4%)和红霉素(93.7%)的抗药性较高。所有分离株均携带 blaTEM。MLST 结果表明,仅鉴定出一个序列型(ST11)。我们的研究结果表明, 不仅在肉鸡养殖场普遍存在,而且在种鸡养殖场也普遍存在。