Son Jigwan, Lee Hyosun, Kim Mincheol, Kim Dong-Uk, Ka Jong-Ok
Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, 08826, Republic of Korea.
Korea Polar Research Institute, Incheon, 21990, Republic of Korea.
Curr Microbiol. 2021 May;78(5):2143-2150. doi: 10.1007/s00284-021-02379-y. Epub 2021 Apr 17.
A novel BTEX degrading bacterial strain, designated ML15P13, was isolated from Arctic soil at the Svalbard Islands, Norway, using an enrichment culture technique. This isolate is Gram-negative, aerobic, motile with multiple flagella at one polar end, and rod-shaped. Growth was observed at 4-35 °C, pH 6.0-8.0, and 0-0.5% (w/v) NaCl. According to 16S rRNA gene analysis, strain ML15P13 was grouped with members of the genus Massilia and closely related to Massilia atriviolacea SOD (98.4%), Massilia violaceinigra B2 (98.3%), Massilia eurypsychrophila B528-3 (97.7%), Massilia glaciei B448-2 (97.7%), and Massilia psychrophila B115-1 (96.6%). Average nucleotide identity, digital DNA-DNA hybridization, and average amino acid identity between genome sequences of strain ML15P13 and the closely related species ranged from 75.8 to 84.3%, from 19.6 ± 1.0 to 21.6 ± 0.3%, and from 68.8 to 71.0%, respectively. The major fatty acids were C, summed feature 3 (C ω6c and/or C ω7c), and summed feature 8 (C ω7c and/or C ω6c). Q-8 was the major ubiquinone. The polar lipid profile showed the presence of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, one unidentified phospholipid, and five unidentified polar lipids. The G + C content of the genomic DNA was 64.2 mol%. Based on the results for genotypic and phenotypic study, we conclude that strain ML15P13 represents a novel species of the genus Massilia, for which the name Massilia aromaticivorans sp. nov. is proposed. The type strain is ML15P13 (= KACC 21773 = JCM 34089).
采用富集培养技术，从挪威斯瓦尔巴群岛的北极土壤中分离出一株新型的BTEX降解细菌菌株，命名为ML15P13。该菌株为革兰氏阴性菌，好氧，具运动性，在一端有多个鞭毛，呈杆状。在4 - 35°C、pH 6.0 - 8.0和0 - 0.5%（w/v）NaCl条件下可观察到其生长。根据16S rRNA基因分析，菌株ML15P13与马赛菌属成员归为一类，与嗜暗马赛菌SOD（98.4%）、紫黑马赛菌B2（98.3%）、广嗜冷马赛菌B528 - 3（97.7%）、冰川马赛菌B448 - 2（97.7%）和嗜冷马赛菌B115 - 1（96.6%）密切相关。菌株ML15P13与密切相关物种的基因组序列之间的平均核苷酸同一性、数字DNA - DNA杂交和平均氨基酸同一性分别为75.8%至84.3%、19.6±1.0%至21.6±0.3%和68.8%至71.0%。主要脂肪酸为C、总和特征3（C ω6c和/或C ω7c）以及总和特征8（C ω7c和/或C ω6c）。泛醌Q - 8是主要的泛醌。极性脂质谱显示存在磷脂酰乙醇胺、磷脂酰甘油、二磷脂酰甘油、一种未鉴定的磷脂和五种未鉴定的极性脂质。基因组DNA的G + C含量为64.2 mol%。基于基因型和表型研究结果，我们得出结论，菌株ML15P13代表马赛菌属的一个新物种，为此提出名称嗜芳烃马赛菌新种。模式菌株为ML15P13（= KACC 21773 = JCM 34089）。
