黄瓜的抗性诱导及氧化锆纳米颗粒对茄科镰孢菌的直接抑菌活性。

Resistance induction in cucumber and direct antifungal activity of zirconium oxide nanoparticles against Rhizoctonia solani.

机构信息

Pesticides Chemistry and Toxicology Department, Faculty of Agriculture, Kafrelsheikh University, 33516, Egypt.

Agricultural Botany Department, Faculty of Agriculture, Kafrelsheikh University, 33516, Egypt.

出版信息

Pestic Biochem Physiol. 2019 Jun;157:230-236. doi: 10.1016/j.pestbp.2019.03.018. Epub 2019 Apr 1.

DOI:10.1016/j.pestbp.2019.03.018

PMID:31153473

Abstract

Zirconium oxide nanoparticles (ZrONPs) were synthesized and evaluated for their ability to induce root rot resistance in cucumber and direct antifungal activity against Rhizoctonia solani. Resistance induction was investigated using real-time polymerase chain reaction (RT-PCR) and the effect of ZrONPs on the growth of cucumber plants was investigated. The results showed that ZrONPs at a concentration level of 100 μg/L significantly inhibited the growth of Rhizoctonia solani (86.6%) relative to untreated control under laboratory conditions. Cucumber plants treated with ZrONPs showed reduction in the severity of root rot disease under greenhouse (34-46%) and field conditions (52-56%) compared with non-treated control plants. Cucumber plants treated with ZrONPs expressed regulatory and defense genes involved in the salicylic acid (SA) and jasmonic acid (JA)/ethylene (ET) signaling pathways with 7-8 folds higher than the control. Treatment of cucumber with ZrONPs and carboxin + thiram significantly improved cucumber growth and yield characters. Therefore, using ZrONPs could be a new strategy to control this pathogen and considered the first report.

摘要

氧化锆纳米粒子（ZrONPs）被合成并评估其诱导黄瓜根腐病抗性和直接抗立枯丝核菌活性的能力。使用实时聚合酶链反应（RT-PCR）研究了抗性诱导，研究了ZrONPs 对黄瓜植物生长的影响。结果表明，在实验室条件下，浓度为 100μg/L 的 ZrONPs 与未处理对照相比，显著抑制了立枯丝核菌的生长（86.6%）。与未处理对照植物相比，温室（34-46%）和田间条件（52-56%）下用 ZrONPs 处理的黄瓜植物根腐病严重程度降低。用 ZrONPs 处理的黄瓜植物表达了与水杨酸（SA）和茉莉酸（JA）/乙烯（ET）信号通路相关的调节和防御基因，是对照的 7-8 倍。用 ZrONPs 和羧菌胺+福美双处理黄瓜显著改善了黄瓜的生长和产量性状。因此，使用 ZrONPs 可能是控制这种病原体的一种新策略，这被认为是首次报道。