State Key Laboratory of Rice Biology and Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Biotechnology, Zhejiang University, 310058 Hangzhou, China.
Institute of Horticulture, Zhejiang Academy of Agricultural Sciences, 310021 Hangzhou, China.
NanoImpact. 2021 Jan;21:100281. doi: 10.1016/j.impact.2020.100281. Epub 2020 Dec 2.
Pestalotiopsis versicolor is a most destructive fungal pathogen that causes twig blight disease in bayberry. For the last seven years, it is difficult to control this pathogen due to its latent infestation mode and its control through chemical fungicides is environmentally corrosive in addition to being costly. In this study, we reported the fungicidal potential of biologically synthesized zirconium oxide nanoparticles (ZrONPs) against P. versicolor for the first time. The strain used for green synthesis of ZrONPs was taxonomically identified as Enterobacter sp. strain RNT10. The production of ZrONPs in reaction mixture was confirmed through UV-vis spectroscopy analysis. Moreover, FTIR, XRD, SEM and TEM analysis showed the presence of capping proteins and crystalline nature of spherical shaped ZrONPs with particle size ranging from 33 to 75 nm. EDX spectra revealed an elemental profile of ZrONPs comprising of Zr (54.40%) and oxygen (43.49%). Biogenic ZrONPs showed substantial antifungal inhibition zones (25.18 ± 1.52 mm) at 20 μg mL concentration against P. versicolor strain XJ27. Moreover, the treatment of 20 μg mL ZrONPs significantly inhibited twig blight in detached leaf assay. Furthermore, imaging through SEM and TEM showed the adverse effects of ZrONPs against P. versicolor in terms of extracellular leakage of DNA and proteins. Overall, this study suggested that biogenic ZrONPs could substitute chemically synthesized antifungal agents with the specific application towards control of twig blight disease in bayberry.
树生枝孢霉是一种极具破坏性的真菌病原体，可引起杨梅的嫩枝枯萎病。在过去的七年中，由于其潜伏的侵染模式以及通过化学杀真菌剂进行控制既具有腐蚀性又成本高昂，因此很难控制这种病原体。在这项研究中，我们首次报道了生物合成的氧化锆纳米粒子（ZrONPs）对树生枝孢霉的杀菌潜力。用于绿色合成 ZrONPs 的菌株被分类鉴定为肠杆菌属 RNT10 菌株。通过紫外-可见光谱分析证实了反应混合物中 ZrONPs 的产生。此外，FTIR、XRD、SEM 和 TEM 分析表明存在封端蛋白和球形 ZrONPs 的结晶特性，其粒径范围为 33 至 75nm。EDX 光谱揭示了 ZrONPs 的元素图谱，其中包含 Zr（54.40%）和氧（43.49%）。生物合成的 ZrONPs 在 20μg mL 浓度下对 P. versicolor 菌株 XJ27 表现出显著的抑菌抑制区（25.18±1.52mm）。此外，20μg mL ZrONPs 的处理显著抑制了离体叶片试验中的嫩枝枯萎病。此外，通过 SEM 和 TEM 成像显示，ZrONPs 对 P. versicolor 产生了不利影响，表现为 DNA 和蛋白质的细胞外泄漏。总体而言，这项研究表明，生物合成的 ZrONPs 可以替代化学合成的抗真菌剂，特别适用于控制杨梅的嫩枝枯萎病。
