Organic Biomolecular Research Group, Faculty of Pharmacy and Bioanalysis, Research Institute, University of Los Andes, Mérida 5101, Venezuela.
ULR7519, Unité Transformations & Agroressources, Université d'Artois-UniLasalle, F-62408 Béthune, France.
Biomolecules. 2021 Apr 19;11(4):605. doi: 10.3390/biom11040605.
Essential oils obtained by hydrodistillation of leaves, flowers, and stems were analyzed by GC and GC/MS techniques revealing myrcene, limonene, β-phellandrene, and sabinene among the main components. The aim of the present study was to evaluate the MDR modulator activity on human MDR1 gene transfected mouse lymphoma cell line and the antimicrobial activity on the essential oils obtained from different parts of the species under investigation. The results revealed that MQL caused a similar increase in the fluorescence activity of the cells at 0.02 μL/mL comparing to the Verapamil value. The antimicrobial assay was carried out according to the disc diffusion method. Five different bacterial strains (, , , AG 100, and AG100A) were treated with the essential oils and the zones of inhibition were determined on TSA plates and TSA agar plates supplemented with Tween 20. MQF and MQL showed activity against , , and AG 100A while MQS was only active against AG 100A on TSA agar plates experiment. In case of TSA agar plates supplemented with 0.1 /% Tween 20 detergent, MQF showed inhibition on , , and AG 100A; MQL was active against , AG 100, AG 100A while MQS was only active against AG 100A.
通过水蒸气蒸馏法从叶片、花朵和茎中提取的精油，经 GC 和 GC/MS 技术分析，发现其中的主要成分有月桂烯、柠檬烯、β-蒎烯和 Sabinene。本研究旨在评估 MQL 对人 MDR1 基因转染的鼠淋巴瘤细胞系的多药耐药调节剂活性，以及对所研究物种不同部位获得的精油的抗菌活性。结果表明，与维拉帕米相比，MQL 在 0.02 μL/mL 时能使细胞荧光活性有相似的增加。采用纸片扩散法进行抗菌试验。用精油处理 5 种不同的细菌菌株（、、、AG100 和 AG100A），并在 TSA 平板和添加 Tween 20 的 TSA 琼脂平板上测定抑菌圈直径。MFQ 和 MQL 对、和 AG100A 有活性，而 MQS 仅在 TSA 琼脂平板实验中对 AG100A 有活性。在添加 0.1 /% Tween 20 清洁剂的 TSA 琼脂平板中，MFQ 对、和 AG100A 有抑制作用；MQL 对、AG100、AG100A 有活性，而 MQS 仅对 AG100A 有活性。
