赞比亚非人灵长类动物感染寨卡病毒的血清学证据。
Serological evidence of Zika virus infection in non-human primates in Zambia.
作者信息
Wastika Christida E, Sasaki Michihito, Yoshii Kentaro, Anindita Paulina D, Hang'ombe Bernard M, Mweene Aaron S, Kobayashi Shintaro, Kariwa Hiroaki, Carr Michael J, Hall William W, Eshita Yuki, Orba Yasuko, Sawa Hirofumi
机构信息
Division of Molecular Pathobiology, Research Center for Zoonosis Control, Hokkaido University, N20, W10, Kita-Ku, Sapporo, 001-0020, Japan.
Laboratory of Public Health, Graduate School of Veterinary Medicine, Hokkaido University, N18, W9, Kita-ku, Sapporo, 060-0818, Japan.
出版信息
Arch Virol. 2019 Aug;164(8):2165-2170. doi: 10.1007/s00705-019-04302-0. Epub 2019 Jun 1.
Zika virus (ZIKV) circulation occurs between non-human primates (NHPs) in a sylvatic transmission cycle. To investigate evidence of flavivirus infection in NHPs in Zambia, we performed a plaque reduction neutralization test (PRNT) to quantify neutralizing antibodies. PRNT revealed that sera from NHPs (African green monkeys and baboons) exhibited neutralizing activity against ZIKV (34.4%; 33/96), whereas a PRNT for yellow fever virus using NHP sera showed no neutralization activity. ZIKV genomic RNA was not detected in splenic tissues from NHPs, suggesting that the presence of anti-ZIKV neutralizing antibodies represented resolved infections. Our evidence suggests that ZIKV is maintained in NHP reservoirs in Zambia.
寨卡病毒（ZIKV）在野生传播循环中于非人灵长类动物（NHPs）之间传播。为调查赞比亚非人灵长类动物中黄病毒感染的证据，我们进行了蚀斑减少中和试验（PRNT）以量化中和抗体。PRNT显示，非人灵长类动物（非洲绿猴和狒狒）的血清对寨卡病毒表现出中和活性（34.4%；33/96），而使用非人灵长类动物血清进行的黄热病病毒PRNT未显示中和活性。在非人灵长类动物的脾脏组织中未检测到寨卡病毒基因组RNA，这表明抗寨卡病毒中和抗体的存在代表感染已消除。我们的证据表明，寨卡病毒在赞比亚的非人灵长类动物宿主中持续存在。
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