Department of Orthopaedic Surgery and Laboratory of Orthopaedic Research, Chosun University Hospital, Gwangju, 61453, South Korea.
Department of Premedical Program, School of Medicine, Chosun University, Gwangju, 61452, South Korea.
Lasers Med Sci. 2020 Feb;35(1):115-120. doi: 10.1007/s10103-019-02801-9. Epub 2019 Jun 1.
Bioluminescence imaging is being increasingly utilized in biological research. However, since the most commonly used firefly luciferase generates relatively weak bioluminescent signals, detection of low numbers of luciferase-expressing cells in vivo is challenging. The weak signal makes it difficult to detect cells located in deep tissues, which is problematic for preclinical research in tumor metastasis. In this study, three different types of fluorophores such as D-luciferin, AkaLumine-HCl, and P800SO3 were compared to evaluate the progression of bone metastasis induced by MDA-MB-231 breast cancer cells in vivo. The fluorescent signals for D-luciferin, AkaLumine-HCl, and P800SO3 were differently detected in the chest and knee joint. In particular, the fluorescence signal of P800SO3 was clearly observed in a section of the ribs, where it pointed out fractured bone fragments by tumor mass. Moreover, the P800SO3 signal from the left knee joint also showed a small bone fragment in the distal femur and was highlighted in the proximal tibia. Using targeted NIR fluorophores, metastatic bone tumors were monitored under the NIR fluorescence imaging system in real time, which enabled the in vivo diagnosis of bone metastasis by providing the location of the metastatic bone tumors.
生物发光成像是在生物研究中越来越多地被利用。然而，由于最常用的萤火虫荧光素酶产生相对较弱的生物发光信号，因此在体内检测低数量的表达荧光素酶的细胞具有挑战性。由于信号较弱，很难检测到位于深部组织中的细胞，这对于肿瘤转移的临床前研究是一个问题。在这项研究中，比较了三种不同类型的荧光团，如 D-荧光素、AkaLumine-HCl 和 P800SO3，以评估 MDA-MB-231 乳腺癌细胞在体内诱导的骨转移的进展。D-荧光素、AkaLumine-HCl 和 P800SO3 的荧光信号在胸部和膝关节中被不同地检测到。特别是，P800SO3 的荧光信号在肋骨的一部分中清晰可见，其中肿瘤块指出了骨折的骨碎片。此外，来自左膝关节的 P800SO3 信号也显示了在远端股骨中的小骨碎片，并在近端胫骨中突出显示。使用靶向近红外荧光团，通过近红外荧光成像系统实时监测转移性骨肿瘤，通过提供转移性骨肿瘤的位置，实现了骨转移的体内诊断。
