Lin Rui, Piao Meiyu, Song Yan
Department of Gastroenterology and Hepatology, General Hospital, Tianjin Medical University, Tianjin, China.
Front Microbiol. 2019 May 16;10:1092. doi: 10.3389/fmicb.2019.01092. eCollection 2019.
Disturbed balance between microbiota, epithelial cells, and resident immune cells within the intestine contributes to inflammatory bowel disease (IBD) pathogenesis. The -induced colitis mouse model has been well documented. This model allows the analysis of host responses to enteric bacteria and facilitates improved understanding of the potential mechanisms of IBD pathogenesis. The current study evaluated the effects of dietary 30 mg/kg quercetin supplementation on -induced experimental colitis in C57BL/6 mice. Following dietary quercetin supplementation, the mice were infected with 5 × 10 CFU , and the pathological effects of were measured. The results showed that quercetin alleviated the effects of -induced colitis, suppressed the production of pro-inflammatory cytokines, such as interleukin (IL)-17, tumor necrosis factor alpha, and IL-6 ( < 0.05), and promoted the production of IL-10 in the colon tissues ( < 0.05). Quercetin supplementation also enhanced the populations of , , and and significantly reduced those of and ( < 0.05). These findings indicate that dietary quercetin exerts therapeutic effects on -induced colitis, probably due to quercetin's ability to suppress pro-inflammatory cytokines and/or modify gut microbiota. Thus, these results suggest that quercetin supplementation is effective in controlling -induced inflammation.
肠道内微生物群、上皮细胞和常驻免疫细胞之间的平衡失调会导致炎症性肠病（IBD）的发病机制。诱导性结肠炎小鼠模型已有充分记录。该模型有助于分析宿主对肠道细菌的反应，并促进对IBD发病机制潜在机制的更好理解。本研究评估了日粮中添加30mg/kg槲皮素对C57BL/6小鼠诱导性实验性结肠炎的影响。日粮补充槲皮素后，小鼠感染5×10CFU，测量其病理效应。结果表明，槲皮素减轻了诱导性结肠炎的影响，抑制了促炎细胞因子如白细胞介素（IL）-17、肿瘤坏死因子α和IL-6的产生（<0.05），并促进了结肠组织中IL-10的产生（<0.05）。补充槲皮素还增加了、、和的数量，并显著减少了和的数量（<0.05）。这些发现表明，日粮中的槲皮素对诱导性结肠炎具有治疗作用，可能是由于槲皮素抑制促炎细胞因子和/或改变肠道微生物群的能力。因此，这些结果表明补充槲皮素对控制诱导性炎症有效。
