Research Center for Prevention of Oral and Dental Diseases, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Department of Hematology & Blood Banking, School of Allied Medical Sciences, Iran University of Medical Sciences, Tehran, 14155-5983, Iran.
Inflammation. 2019 Oct;42(5):1622-1629. doi: 10.1007/s10753-019-01023-z.
Periodontitis as a chronic inflammatory disease leads to the destruction of the supportive tissues of affected teeth. Crosstalk between periodontitis and the host immune system plays a crucial role in the pathogenesis of this disease. Since polyphenol components such as silymarin and resveratrol have anti-bacterial and anti-inflammatory effects on periodontal tissues, the purpose of this study was to investigate the anti-histaminic effects of silymarin and resveratrol on human gingival fibroblasts (HGFs). HGFs were treated with a concentration of silymarin or resveratrol (100 μg/ml) and a combination of these two polyphenols (50/100 or 100/200 μg/ml silymarin/resveratrol). The effect of silymarin and resveratrol on cell viability was assessed by MTT assay. Also, HGFs were treated with silymarin and/or resveratrol and were stimulated by histamine. The levels of interleukin-6 (IL-6), interleukin-8 (IL-8), tumor necrosis factor alpha (TNF-α), and tissue plasminogen activator 1 (TPA-1) were assessed by enzyme-linked immunosorbent assay (ELISA). After treatment with silymarin, the viability of fibroblast cells significantly increased, whereas treatment with resveratrol and combinations of these flavonoids (silymarin 50 μg/ml and resveratrol 100 μg/ml) did not have any significant effect on cell viability after 24 h. Treatment with 100/200 μg/ml silymarin/resveratrol significantly decreased the cell viability after 48 h. Resveratrol inhibited histamine-induced IL-6 secretion by HGFs significantly, whereas silymarin showed significant effect on TNF-α. A blend of silymarin and resveratrol displayed more valuable results. In conclusion, combination of resveratrol and silymarin could significantly inhibit inflammatory effects of histamine on cultured HGFs by reduction of IL-6, IL-8, TPA-1, and TNF-α.
牙周炎是一种慢性炎症性疾病，会导致受影响牙齿的支持组织破坏。牙周炎和宿主免疫系统之间的串扰在这种疾病的发病机制中起着至关重要的作用。由于水飞蓟素和白藜芦醇等多酚成分对牙周组织具有抗菌和抗炎作用，因此本研究旨在研究水飞蓟素和白藜芦醇对人牙龈成纤维细胞（HGFs）的抗组胺作用。用水飞蓟素或白藜芦醇（100μg/ml）浓度以及这两种多酚（50/100 或 100/200μg/ml 水飞蓟素/白藜芦醇）的混合物处理 HGFs。通过 MTT 测定法评估水飞蓟素和白藜芦醇对细胞活力的影响。此外，用水飞蓟素和/或白藜芦醇处理 HGFs，并通过组胺刺激。通过酶联免疫吸附试验（ELISA）评估白细胞介素-6（IL-6）、白细胞介素-8（IL-8）、肿瘤坏死因子-α（TNF-α）和组织纤溶酶原激活物 1（TPA-1）的水平。用水飞蓟素处理后，成纤维细胞的活力显着增加，而用白藜芦醇和这些类黄酮（水飞蓟素 50μg/ml 和白藜芦醇 100μg/ml）的混合物处理 24 小时后对细胞活力没有任何显着影响。48 小时后，用 100/200μg/ml 水飞蓟素/白藜芦醇处理显着降低了细胞活力。白藜芦醇显着抑制 HGFs 中组胺诱导的 IL-6 分泌，而水飞蓟素对 TNF-α 显示出显着作用。水飞蓟素和白藜芦醇的混合物显示出更有价值的结果。总之，白藜芦醇和水飞蓟素的组合通过降低 IL-6、IL-8、TPA-1 和 TNF-α，可显着抑制组胺对培养的 HGFs 的炎症作用。
