Yadav Vineeta, Majumder Shahana
Department of Biotechnology, Sharda University, Knowledge Park III, G. Noida, 201306 India.
Virusdisease. 2019 Jun;30(2):311-314. doi: 10.1007/s13337-018-0505-x. Epub 2019 Jan 5.
The complete genome sequence of a garlic common latent virus (GarCLV) isolate collected from NHRDF, Karnal, India was determined. The whole genome of this GarCLV isolate consists of 8573 nucleotides, excluding the 3'-end poly-A tail. The sequence is 91-93% similar to rest of the five whole genome sequences available till date. It contains six open reading frames (ORFs) which encode polypeptide of 219 kDa, 25 kDa, 11 kDa, 7 kDa, 32.74 kDa and 15 kDa from ORF1, ORF2, ORF3, ORF4, ORF5 and ORF6 respectively. The phylogenetic study based on coat protein sequence demonstrated high diversity present among Indian population of GarCLV.
测定了从印度卡纳尔国家植物遗传资源局（NHRDF）收集的一株大蒜普通潜隐病毒（GarCLV）分离物的全基因组序列。该GarCLV分离物的全基因组由8573个核苷酸组成，不包括3'端的多聚A尾。该序列与迄今为止已有的其他五个全基因组序列的相似性为91%-93%。它包含六个开放阅读框（ORF），分别从ORF1、ORF2、ORF3、ORF4、ORF5和ORF6编码219 kDa、25 kDa、11 kDa、7 kDa、32.74 kDa和15 kDa的多肽。基于外壳蛋白序列的系统发育研究表明，GarCLV的印度种群中存在高度多样性。
