Eaton S, Calame K
Department of Microbiology, University of California, Los Angeles 90024.
Proc Natl Acad Sci U S A. 1987 Nov;84(21):7634-8. doi: 10.1073/pnas.84.21.7634.
Sequences required for the function of the mouse V1 immunoglobulin heavy chain variable-region (VH) promoter were identified by transient transfection of the normal and mutated promoters into plasmacytoma cells. Our results identify four regions required for normal promoter function: (i) the octamer ATGCAAAT, previously identified by others; (ii) a heptamer, CTAATGA; (iii) a pyrimidine-rich region; and (iv) a region between positions -125 and -251 relative to the transcription start site. Sequence analysis of 19 mouse and human VH 5' flanking regions shows that the heptamer and pyrimidine stretch are strongly conserved. We have also demonstrated that the octamer functions in an orientation independent manner in the VH promoter.
通过将正常和突变的启动子瞬时转染到浆细胞瘤细胞中，确定了小鼠V1免疫球蛋白重链可变区（VH）启动子功能所需的序列。我们的结果确定了正常启动子功能所需的四个区域：（i）八聚体ATGCAAAT，先前已被其他人鉴定；（ii）七聚体CTAATGA；（iii）富含嘧啶的区域；以及（iv）相对于转录起始位点在-125至-251位之间的区域。对19个小鼠和人类VH 5'侧翼区域的序列分析表明，七聚体和嘧啶延伸序列高度保守。我们还证明了八聚体在VH启动子中以方向独立的方式发挥作用。
