新种和新种，从环境来源中分离出的致病性群组的两个新物种。
sp. nov. and sp. nov., two new species of the pathogenic group isolated from environmental sources.
机构信息
Epidemiology of Microbial Diseases, School of Public Health, Yale University, New Haven, CT, USA.
Present address: Instituto Nacional de Investigación Agropecuaria, Estación Experimental La Estanzuela, Colonia, Uruguay and Unidad Mixta Pasteur + INIA (UMPI), Institut Pasteur de Montevideo/Instituto Nacional de Investigación Agropecuaria, Montevideo, Uruguay.
出版信息
Int J Syst Evol Microbiol. 2020 Mar;70(3):1450-1456. doi: 10.1099/ijsem.0.003480.
Four spirochetes (F1, B21, Yale and AMB6-RJ) were isolated from environmental sources: F1 and B21 from soils of an urban slum community in Salvador (Brazil), Yale from river water in New Haven, Connecticut (USA) and AMB6-RJ from a pond in a horse farm in Rio de Janeiro (Brazil). Isolates were helix-shaped, aerobic, highly motile and non-virulent in a hamster model of infection. Draft genomes of the strains were obtained and analysed to determine the relatedness to other species of the genus . The analysis of 498 core genes showed that strains F1/B21 and Yale/AMB6-RJ formed two distinct phylogenetic clades within the 'Pathogens' group (group I). The average nucleotide identity (ANI) values of strains F1/B21 and Yale/AMB6-RJ to other previously described species were below <84 % and <82 %, respectively, which confirmed that these isolates should be classified as representatives of two novel species. Therefore, we propose sp. nov. and sp. nov. as new species in the genus . The type strains are F1 (=ATCC-TSD-163=KIT0259=CLEP00287) and Yale (=ATCC-TDS-162=KIT0258=CLEP00288), respectively.
从环境源中分离出了 4 株螺旋体（F1、B21、耶鲁和 AMB6-RJ）：F1 和 B21 来自巴西萨尔瓦多城市贫民窟社区的土壤，耶鲁来自美国康涅狄格州纽黑文的河水，AMB6-RJ 来自巴西里约热内卢一个马场的池塘。分离株呈螺旋形，需氧，高度运动，在仓鼠感染模型中无毒性。获得了这些菌株的草图基因组，并进行了分析以确定与属内其他物种的亲缘关系。对 498 个核心基因的分析表明，F1/B21 和 Yale/AMB6-RJ 株在“病原体”组（组 I）内形成两个不同的系统发育分支。F1/B21 和 Yale/AMB6-RJ 株与其他先前描述的物种的平均核苷酸同一性（ANI）值分别低于<84%和<82%，这证实这些分离株应归类为两个新种的代表。因此，我们提议 为新属的新种。其模式株分别为 F1（=ATCC-TSD-163=KIT0259=CLEP00287）和耶鲁（=ATCC-TDS-162=KIT0258=CLEP00288）。
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