Wang Hao, Ling Yu, Shan Tongling, Yang Shixing, Xu Hui, Deng Xutao, Delwart Eric, Zhang Wen
Department of Microbiology, School of Medicine, Jiangsu University, 310 Xuefu Road, Zhenjiang, Jiangsu, China.
Department of Swine Infectious Disease, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 518 Ziyue Road, Shanghai, China.
Virus Evol. 2019 Jun 8;5(1):vez013. doi: 10.1093/ve/vez013. eCollection 2019 Jan.
Nineteen families of phages infecting bacteria or archaea are currently recognized by the International Committee on Taxonomy of Viruses (ICTV). Of these, only two have single-stranded DNA genomes, namely and e. The distribution, genetic characteristics, and ecological roles of remain largely under explored. Here, using viral metagenomics, we investigate the intestinal virome from human and twenty-four species of animals, as well as freshwater samples, containing abundant sequence reads showing similarity to the . Eight hundred and sixty complete or near complete -related genomes were generated, showing high levels of co-infections and sequence divergence. Sequence comparison and phylogenetic analysis showed that the subfamily was highly prevalent and that some strains may qualify as new subfamilies. This study significantly augments our knowledge of the genetic diversity, genome evolution, and distribution in animal species of members of the family
目前，国际病毒分类委员会（ICTV）已识别出19个感染细菌或古菌的噬菌体家族。其中，只有两个家族具有单链DNA基因组，即 和 。 的分布、遗传特征和生态作用在很大程度上仍有待探索。在这里，我们使用病毒宏基因组学方法，研究了来自人类和24种动物的肠道病毒组，以及淡水样本，这些样本中含有大量与 相似的序列读数。我们生成了860个完整或近乎完整的与 相关的基因组，显示出高水平的共感染和序列差异。序列比较和系统发育分析表明， 亚家族非常普遍，并且一些菌株可能符合新亚家族的标准。这项研究显著增加了我们对该噬菌体家族成员在动物物种中的遗传多样性、基因组进化和分布的了解。
