钆在糖尿病大鼠模型中经钆喷替酸葡甲胺、钆贝葡胺和钆特酸葡胺给药后的脑内蓄积和清除。
Gadolinium Retention and Clearance in the Diabetic Brain after Administrations of Gadodiamide, Gadopentetate Dimeglumine, and Gadoterate Meglumine in a Rat Model.
机构信息
Department of Radiology, The First Affiliated Hospital, China Medical University, Shenyang, Liaoning 110001, China.
Key Laboratory of Imaging Diagnosis and Interventional Radiology of Liaoning Province, Shenyang, Liaoning 110001, China.
出版信息
Biomed Res Int. 2019 May 5;2019:3901907. doi: 10.1155/2019/3901907. eCollection 2019.
PURPOSE
To evaluate gadolinium (Gd) retention and clearance in the brain of diabetic rats after administrations of gadodiamide, gadopentetate dimeglumine, and gadoterate meglumine.
MATERIALS AND METHODS
Both diabetic rats (n = 52) and normal rats (n = 52) intravenously received 20 injections of 0.6 mmol Gd/kg gadodiamide, gadopentetate dimeglumine, gadoterate meglumine, or saline. Both diabetic rats and normal rats were divided into 2 subgroups of 24 and 28 rats for the 7-day and 42-day evaluations (i.e., they were sacrificed at 7 days (n = 6 per group) and 42 days (n = 7 per group)), respectively, after the last injection. For the 7-day subgroup, 6 rats were euthanized for inductively coupled plasma mass spectrometry (ICP-MS) analysis. For the 42-day subgroup, 6 rats underwent T1-weighted magnetic resonance imaging (MRI) and ICP-MS, and 1 rat was analyzed by transmission electron microscopy (TEM).
RESULTS
The T1 enhancements in the deep cerebellar nuclei (DCNs) of diabetic rats were lower than those of normal rats in both linear Gd-based contrast agent (GBCA) groups (p < 0.05). The average Gd concentrations in the brains of diabetic rats were significantly lower than those of healthy rats in both the short-term groups and long-term groups ( 0.05). The highest Gd retentions were in the olfactory bulb, DCN, and striatum with gadodiamide. Compared with the results obtained 7 days after the last injection, the residual Gd concentrations of the 42-day subgroups in the brains of diabetic rats showed no significant difference in both linear GBCA groups (0.05).
CONCLUSIONS
Compared with normal rats, the diabetic status decreased the residual Gd concentrations in the brain after multiple administrations of gadodiamide, gadopentetate dimeglumine, and gadoterate meglumine. The clearable fraction of Gd in the brain was eliminated faster in diabetic rats than in normal rats.
目的
评估糖尿病大鼠在静脉注射钆喷酸葡胺、钆喷替酸葡甲胺和钆特酸葡胺后脑内的钆蓄积和清除情况。
材料与方法
52 只糖尿病大鼠和 52 只正常大鼠分别接受 20 次 0.6mmol/kg 钆喷酸葡胺、钆喷替酸葡甲胺和钆特酸葡胺静脉注射或生理盐水注射。将糖尿病大鼠和正常大鼠分别分为 7 天和 42 天评价的 2 个亚组（即末次注射后第 7 天（每组 6 只）和第 42 天（每组 7 只）处死）。7 天亚组中，6 只大鼠用于电感耦合等离子体质谱（ICP-MS）分析。42 天亚组中，6 只大鼠进行 T1 加权磁共振成像（MRI）和 ICP-MS 检查，1 只大鼠进行透射电子显微镜（TEM）检查。
结果
糖尿病大鼠深部小脑核（DCN）的 T1 增强值在所有线性 GBCA 组中均低于正常大鼠（p<0.05）。短期和长期组中，糖尿病大鼠脑内的平均 Gd 浓度均显著低于健康大鼠（p<0.05）。在所有 GBCA 中，Gd 蓄积最高的部位是嗅球、DCN 和纹状体。与末次注射后 7 天相比，糖尿病大鼠脑内 42 天亚组的残留 Gd 浓度在所有线性 GBCA 组中均无明显差异（p>0.05）。
结论
与正常大鼠相比，糖尿病状态降低了多次静脉注射钆喷酸葡胺、钆喷替酸葡甲胺和钆特酸葡胺后大鼠脑内的残留 Gd 浓度。糖尿病大鼠脑内 Gd 的可清除部分比正常大鼠清除得更快。
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