Schmitz Laura, Ferrari Nina, Schwiertz Andreas, Rusch Kerstin, Woestmann Ulrich, Mahabir Esther, Graf Christine
1Institute of Movement and Neuroscience, Department of Movement and Health Promotion, German Sport University Cologne, Am Sportpark Müngersdorf 6, Cologne, Germany.
5Cologne Center for Prevention in Childhood and Youth/Heart Center Cologne, University Hospital of Cologne, Kerpener Str. 62, Cologne, Germany.
Pilot Feasibility Stud. 2019 Jun 8;5:76. doi: 10.1186/s40814-019-0459-9. eCollection 2019.
The human microbiota has a broad range of functions contributing to metabolic processes and the activities of our immune system. Its influence on health, well-being and chronic diseases are discussed in various studies. The intestinal microbiota and the mucosal integrity are influenced by diet, environment and other lifestyle factors, including physical activity. There are correlations between cardiorespiratory fitness and important markers of intestinal health. However, data linking endurance exercise to microbiota composition are sparse. Many endurance athletes take probiotics to reduce gastrointestinal symptoms linked to exercise or immunosuppression, but the longitudinal data is insufficient.This randomised, controlled cross-over pilot study will examine the impact of specific endurance training and probiotic supplementation on the intestinal microbiota and mucosa in healthy, athletic students.
The aim of this pilot study is to elucidate the impact of physical activity on the intestinal microbiota and mucosa with regard to the effects of a probiotic supplementation.
In this pilot study, thirty non-specifically trained student athletes will participate in an intervention consisting of a two-week rest (baseline) period, a four-week exercise programme and a four-week probiotic intervention using SymbioLactComp®. The exercise programme consists of three 60-min running workouts per week at 70-85% of the peak heart rate (HRpeak). Primary endpoint of this pilot study is the feasibility and practicality of the intervention as well as a sample size estimation. Furthermore, anthropometric measurements and information on nutrition and lifestyle will be obtained. The peak oxygen uptake (VOpeak) and peak heart rate (HRpeak) (determined during a shuttle run test) as well as selected blood and saliva parameters (haemogram, cytokines) will be evaluated. Changes to the intestinal microbiota will be analysed by stool diagnostics (KyberKompaktPRO®, KyberPlus®). The potential changes may include microbiota composition, bacterial metabolites and mucosa- and immune markers.
Results will be used for the design of a main randomised controlled trial with a larger collective based on feasibility, validity and sample size estimation as well as the potential effects of endurance exercise on intestinal microbiota and mucosa. Evidence-based information of an exercise-altered microbiota could be of importance for the prevention and therapy of intestinal or immune disorders.
German Clinical Trials Register: DRKS00011108. Retrospectively registered on 28 November 2016.
人类微生物群具有广泛的功能，对代谢过程和我们免疫系统的活动有贡献。其对健康、幸福和慢性疾病的影响在各种研究中都有讨论。肠道微生物群和黏膜完整性受饮食、环境和其他生活方式因素影响，包括体育活动。心肺适能与肠道健康的重要标志物之间存在关联。然而，将耐力运动与微生物群组成联系起来的数据很少。许多耐力运动员服用益生菌以减轻与运动或免疫抑制相关的胃肠道症状，但纵向数据不足。这项随机、对照交叉试验性研究将考察特定耐力训练和补充益生菌对健康、爱运动学生的肠道微生物群和黏膜的影响。
这项试验性研究的目的是阐明体育活动对肠道微生物群和黏膜的影响以及补充益生菌的效果。
在这项试验性研究中，30名未经专门训练的学生运动员将参与一项干预，包括为期两周的休息（基线）期、为期四周的运动计划以及为期四周使用SymbioLactComp®的益生菌干预。运动计划包括每周三次60分钟的跑步训练，强度为最高心率（HRpeak）的70 - 85%。这项试验性研究的主要终点是干预的可行性和实用性以及样本量估计。此外，将获取人体测量数据以及营养和生活方式方面的信息。将评估最大摄氧量（VOpeak）和最高心率（HRpeak）（在穿梭跑测试中测定）以及选定的血液和唾液参数（血常规、细胞因子）。将通过粪便诊断（KyberKompaktPRO®、KyberPlus®）分析肠道微生物群的变化。潜在变化可能包括微生物群组成、细菌代谢产物以及黏膜和免疫标志物。
研究结果将用于基于可行性、有效性、样本量估计以及耐力运动对肠道微生物群和黏膜的潜在影响，设计一项针对更大样本群体的主要随机对照试验。运动改变微生物群的循证信息可能对肠道或免疫疾病的预防和治疗具有重要意义。
德国临床试验注册中心：DRKS00011108。于2016年11月28日追溯注册。
