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本文引用的文献

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Genomic, phylogenetic and catabolic re-assessment of the Pseudomonas putida clade supports the delineation of Pseudomonas alloputida sp. nov., Pseudomonas inefficax sp. nov., Pseudomonas persica sp. nov., and Pseudomonas shirazica sp. nov.基因组学、系统发育学和分解代谢分析对假单胞菌属假单胞菌群的再评估支持了假单胞菌属新种的划分，即恶臭假单胞菌、无效假单胞菌、梨形假单胞菌和设拉子假单胞菌。
Syst Appl Microbiol. 2019 Jul;42(4):468-480. doi: 10.1016/j.syapm.2019.04.004. Epub 2019 May 2.
2
CRISPR interference-mediated gene regulation in Pseudomonas putida KT2440.恶臭假单胞菌KT2440中CRISPR干扰介导的基因调控
Microb Biotechnol. 2020 Jan;13(1):210-221. doi: 10.1111/1751-7915.13382. Epub 2019 Feb 22.
3
Fermentative Production of -Methylglutamate From Glycerol by Recombinant .重组菌从甘油中发酵生产α-甲基谷氨酸
Front Bioeng Biotechnol. 2018 Nov 9;6:159. doi: 10.3389/fbioe.2018.00159. eCollection 2018.
4
Refactoring the upper sugar metabolism of Pseudomonas putida for co-utilization of cellobiose, xylose, and glucose.重构恶臭假单胞菌的上层糖代谢途径以共利用纤维二糖、木糖和葡萄糖。
Metab Eng. 2018 Jul;48:94-108. doi: 10.1016/j.ymben.2018.05.019. Epub 2018 Jun 2.
5
Solvent Tolerance in Bacteria: Fulfilling the Promise of the Biotech Era?溶剂耐受性在细菌中的应用：能否兑现生物技术时代的承诺？
Trends Biotechnol. 2018 Oct;36(10):1025-1039. doi: 10.1016/j.tibtech.2018.04.007. Epub 2018 May 16.
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Pseudomonas putida as a functional chassis for industrial biocatalysis: From native biochemistry to trans-metabolism.铜绿假单胞菌作为工业生物催化的功能底盘：从天然生物化学到转代谢。
Metab Eng. 2018 Nov;50:142-155. doi: 10.1016/j.ymben.2018.05.005. Epub 2018 May 16.
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Markerless gene knockout and integration to express heterologous biosynthetic gene clusters in Pseudomonas putida.无标记基因敲除和整合表达异源生物合成基因簇在恶臭假单胞菌。
Metab Eng. 2018 May;47:463-474. doi: 10.1016/j.ymben.2018.05.003. Epub 2018 May 8.
8
Genome editing and transcriptional repression in Pseudomonas putida KT2440 via the type II CRISPR system.通过 II 型 CRISPR 系统在恶臭假单胞菌 KT2440 中进行基因组编辑和转录抑制。
Microb Cell Fact. 2018 Mar 13;17(1):41. doi: 10.1186/s12934-018-0887-x.
9
Genetic tools for reliable gene expression and recombineering in Pseudomonas putida.用于在恶臭假单胞菌中可靠基因表达和基因重组的遗传工具。
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10
Eliminating a global regulator of carbon catabolite repression enhances the conversion of aromatic lignin monomers to muconate in KT2440.消除碳分解代谢物阻遏的全局调控因子可增强KT2440中芳香族木质素单体向粘康酸的转化。
Metab Eng Commun. 2017 May 31;5:19-25. doi: 10.1016/j.meteno.2017.05.002. eCollection 2017 Dec.

恶臭假单胞菌 KT2440 已通过 HV1 认证，而非 GRAS。

Pseudomonas putida KT2440 is HV1 certified, not GRAS.

机构信息

Laboratory of Systems and Synthetic Biology, Wageningen University and Research Centre, Stippeneng 4, 6708, Wageningen, The Netherlands.

Lifeglimmer GmbH, Markelstr. 38, 12163, Berlin, Germany.

出版信息

Microb Biotechnol. 2019 Sep;12(5):845-848. doi: 10.1111/1751-7915.13443. Epub 2019 Jun 14.

DOI:10.1111/1751-7915.13443
PMID:31199068
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6680625/
Abstract

Pseudomonas putida is rapidly becoming a workhorse for industrial production due to its metabolic versatility, genetic accessibility and stress-resistance properties. The P. putida strain KT2440 is often described as Generally Regarded as Safe, or GRAS, indicating the strain is safe to use as food additive. This description is incorrect. P. putida KT2440 is classified by the FDA as HV1 certified, indicating it is safe to use in a P1 or ML1 environment.

摘要

铜绿假单胞菌由于其代谢多样性、遗传可及性和抗应激特性，迅速成为工业生产的得力工具。铜绿假单胞菌菌株 KT2440 通常被描述为“一般认为是安全的”，或 GRAS，这表明该菌株可以安全地用作食品添加剂。这种描述是不正确的。铜绿假单胞菌 KT2440 被 FDA 归类为 HV1 认证，表明其可以在 P1 或 ML1 环境中安全使用。