Department of Medicine, Division of Infectious Diseases, University of British Columbia, Vancouver, BC, Canada.
Chemical and Food Engineering Department, Universidad de las Americas Puebla, San Andres Cholula, Puebla 72810, Mexico.
Biomed Res Int. 2019 May 7;2019:1639726. doi: 10.1155/2019/1639726. eCollection 2019.
Essential oils (EOs) are natural products composed of a mixture of volatile and aromatic compounds extracted from different parts of plants that have shown antimicrobial activities against pathogens. In this study, EOs extracted from (Myrtaceae) and (Lamiaceae) were assessed for their antimicrobial activities using a panel of pathogenic Gram-positive, Gram-negative, and fungal strains. The antimicrobial activity was measured by the minimal inhibitory concentration required for the growth inhibition of the microorganisms. The cytotoxicity of the EOs was tested using the model of human-derived macrophage THP-1 cells. In addition, an inflammatory response was evaluated using the anti-inflammatory cytokine IL-10 and the proinflammatory cytokines IL-6 and TNF-. Results showed that both EOs had antimicrobial activity and different pathogens were exposed to concentrations ranging between 600 and 2000 g/mL. In addition, the EOs showed no inflammatory activity when exposed to human macrophages, but a potent anti-inflammatory activity was measured when the oil from was exposed to macrophages. This study demonstrates that the use of EOs is an effective alternative for pathogenic bacterial and fungal control, alone or in combination with antibiotic therapy. Moreover, the oil extracted from could be used as potent anti-inflammatory agent.
精油（EOs）是从植物不同部位提取的挥发性和芳香化合物混合物，具有抗病原体的抗菌活性。在这项研究中，评估了从桃金娘科和唇形科提取的精油对一系列致病性革兰氏阳性、革兰氏阴性和真菌菌株的抗菌活性。抗菌活性通过微生物生长抑制所需的最小抑制浓度来衡量。使用人源性巨噬细胞 THP-1 细胞模型测试了精油的细胞毒性。此外，还使用抗炎细胞因子 IL-10 和促炎细胞因子 IL-6 和 TNF- 评估了炎症反应。结果表明，两种精油均具有抗菌活性，不同的病原体暴露于浓度在 600 至 2000 g/mL 之间的精油中。此外，当暴露于人巨噬细胞时，精油没有炎症活性，但当暴露于桃金娘科的油时，测量到了强大的抗炎活性。这项研究表明，单独或与抗生素治疗联合使用精油是控制致病性细菌和真菌的有效替代方法。此外，从桃金娘科提取的油可用作有效的抗炎剂。
