College of plant protection, Anhui Agricultural University, 130 West of Changjiang Road, Hefei 230036, Anhui, China.
School of life sciences, Anhui Agricultural University, 130 West of Changjiang Road, Hefei 230036, Anhui, China.
Int J Mol Sci. 2019 Jun 14;20(12):2908. doi: 10.3390/ijms20122908.
With the improper application of fungicides, begins to develop resistance to fungicides, and biological control is one of the potential ways to control it. We screened two strains of ; JDF3 and RSS-1, which had an efficient inhibitory effect on They could inhibit mycelial growth, the germination of the cysts, and the swimming of the motile zoospores. To elucidate the response of under the stress of and , and the molecular mechanism of biological control, comparative transcriptome analysis was applied. Transcriptome analysis revealed that the expression gene of showed significant changes, and a total of 1616 differentially expressed genes (DEGs) were detected. They participated in two major types of regulation, namely "specificity" regulation and "common" regulation. They might inhibit the growth of mainly by inhibiting the activity of ribosome. A pot experiment indicated that and enhanced the resistance of soybean to , and their control effects of them were 70.7% and 65.5%, respectively. In addition, fermentation broth could induce an active oxygen burst, NO production, callose deposition, and lignification. could also stimulate the systemic to develop the resistance of soybean by lignification, and phytoalexin.
由于杀菌剂的不当使用，开始对杀菌剂产生抗性，而生物防治是控制它的潜在方法之一。我们筛选了两种菌株；JDF3 和 RSS-1，它们对有高效的抑制作用。它们可以抑制菌丝生长、胞囊的萌发和游动游孢子的游动。为了阐明在和胁迫下的反应，以及生物防治的分子机制，我们应用了比较转录组分析。转录组分析显示，的表达基因显示出显著的变化，共检测到 1616 个差异表达基因（DEGs）。它们参与了两种主要的调节类型，即“特异性”调节和“共同”调节。它们可能主要通过抑制核糖体的活性来抑制的生长。盆栽试验表明，和增强了大豆对的抗性，它们的防治效果分别为 70.7%和 65.5%。此外，发酵液可以诱导活性氧爆发、NO 产生、胼胝质沉积和木质化。还可以通过木质化和植保素来刺激系统发育大豆的抗性。
