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基因组和转录组分析以阐明XJ5对……的生物防治机制

Genome and Transcriptome Analysis to Elucidate the Biocontrol Mechanism of XJ5 against .

作者信息

Mu Fan, Chen Xu, Fu Zhenxin, Wang Xue, Guo Jiexin, Zhao Xiaojun, Zhang Baojun

机构信息

Shanxi Key Laboratory of Integrated Pest Management in Agriculture, College of Plant Protection, Shanxi Agricultural University, Jinzhong 030801, China.

出版信息

Microorganisms. 2023 Aug 10;11(8):2055. doi: 10.3390/microorganisms11082055.

DOI:10.3390/microorganisms11082055
PMID:37630615
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10459136/
Abstract

Early blight, caused by , is an important disease affecting tomatoes. Biological control offers an environmentally friendly approach to controlling pathogens. Herein, we identified a strain XJ5 and investigated its biocontrol mechanism against . growth was significantly inhibited by XJ5, with the inhibition rate of cell-free culture supernatants reaching 82.3%. Furthermore, XJ5 crude protein extracts inhibited conidia germination and altered the mycelial morphology of . To uncover the potential biocontrol mechanism of XJ5, we analyzed its genome sequence and transcriptome. The genome of XJ5 comprised a 4.16 Mb circular chromosome and two circular plasmids. A total of 13 biosynthetic gene clusters and 127 genes encoding hydrolases were identified, suggestive of the ability of XJ5 to secrete antagonistic secondary metabolites and hydrolases. Transcript analysis revealed 174 differentially expressed genes on exposing to XJ5 crude protein extracts. The expression of genes related to chitin and mannose synthesis was downregulated, indicating that XJ5 metabolites may impact chitin and mannose synthesis in . Overall, these findings enhance our understanding of the interactions between and phytopathogens and pave the way for the agricultural application of this promising biocontrol agent.

摘要

早疫病由[病原体名称缺失]引起，是影响番茄的一种重要病害。生物防治为控制病原体提供了一种环境友好的方法。在此，我们鉴定出一株XJ5菌株，并研究了其对[病原体名称缺失]的生物防治机制。XJ5显著抑制了[病原体名称缺失]的生长，无细胞培养上清液的抑制率达到82.3%。此外，XJ5粗蛋白提取物抑制了分生孢子萌发并改变了[病原体名称缺失]的菌丝形态。为了揭示XJ5潜在的生物防治机制，我们分析了其基因组序列和转录组。XJ5的基因组由一条4.16 Mb的环状染色体和两个环状质粒组成。共鉴定出13个生物合成基因簇和127个编码水解酶的基因，表明XJ5具有分泌拮抗性次生代谢产物和水解酶的能力。转录分析显示，在[病原体名称缺失]暴露于XJ5粗蛋白提取物时，有174个差异表达基因。与几丁质和甘露糖合成相关的基因表达下调，表明XJ5代谢产物可能影响[病原体名称缺失]中的几丁质和甘露糖合成。总体而言，这些发现增进了我们对[病原体名称缺失]与植物病原体之间相互作用的理解，并为这种有前景的生物防治剂的农业应用铺平了道路。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/935d/10459136/58f8bec90dcc/microorganisms-11-02055-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/935d/10459136/adb50906fef6/microorganisms-11-02055-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/935d/10459136/a53b9949b37a/microorganisms-11-02055-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/935d/10459136/e8f9719f1995/microorganisms-11-02055-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/935d/10459136/7f68d5fc625e/microorganisms-11-02055-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/935d/10459136/3d266cc5eb5e/microorganisms-11-02055-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/935d/10459136/60406f9d7e0b/microorganisms-11-02055-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/935d/10459136/58f8bec90dcc/microorganisms-11-02055-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/935d/10459136/adb50906fef6/microorganisms-11-02055-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/935d/10459136/a53b9949b37a/microorganisms-11-02055-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/935d/10459136/e8f9719f1995/microorganisms-11-02055-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/935d/10459136/7f68d5fc625e/microorganisms-11-02055-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/935d/10459136/3d266cc5eb5e/microorganisms-11-02055-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/935d/10459136/60406f9d7e0b/microorganisms-11-02055-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/935d/10459136/58f8bec90dcc/microorganisms-11-02055-g007.jpg

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