Joint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou University, Yangzhou 225009, China.
Jiangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou 225009, China.
Int J Mol Sci. 2019 Jun 18;20(12):2969. doi: 10.3390/ijms20122969.
is a zoonotic pathogen that causes great economic losses to the swine industry and severe threats to public health. A better understanding of its physiology would contribute to the control of its infections. Although copper is an essential micronutrient for life, it is toxic to cells when present in excessive amounts. Herein, we provide evidence that CopA is required for resistance to copper toxicity. Quantitative PCR analysis showed that expression was specifically induced by copper. Growth curve analyses and spot dilution assays showed that the Δ mutant was defective in media supplemented with elevated concentrations of copper. Spot dilution assays also revealed that CopA protected against the copper-induced bactericidal effect. Using inductively coupled plasma-optical emission spectroscopy, we demonstrated that the role of CopA in copper resistance was mediated by copper efflux. Collectively, our data indicated that CopA protects against the copper-induced bactericidal effect via copper efflux.
是一种人畜共患病病原体，它会给养猪业造成巨大的经济损失，并严重威胁公共卫生。更好地了解其生理学特性将有助于控制其感染。尽管铜是生命所必需的微量元素，但当铜含量过高时，它对细胞是有毒的。在此，我们提供的证据表明 CopA 是抵抗铜毒性所必需的。定量 PCR 分析表明，表达受铜的特异性诱导。生长曲线分析和点稀释试验表明，Δ突变体在补充高浓度铜的培养基中生长不良。点稀释试验还表明 CopA 可保护免受铜诱导的杀菌作用。使用电感耦合等离子体 - 光学发射光谱法，我们证明了 CopA 在铜抗性中的作用是通过铜外排介导的。总的来说，我们的数据表明 CopA 通过铜外排来保护免受铜诱导的杀菌作用。
