Joint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou University, Yangzhou 225009, China.
Jiangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou 225009, China.
Genes (Basel). 2020 Sep 2;11(9):1035. doi: 10.3390/genes11091035.
is a zoonotic pathogen causing serious infections in swine and humans. Although metals are essential for life, excess amounts of metals are toxic to bacteria. Transcriptome-level data of the mechanisms for resistance to metal toxicity in are available for no metals other than zinc. Herein, we explored the transcriptome-level changes in in response to ferrous iron and cobalt toxicity by RNA sequencing. Many genes were differentially expressed in the presence of excess ferrous iron and cobalt. Most genes in response to cobalt toxicity showed the same expression trends as those in response to ferrous iron toxicity. qRT-PCR analysis of the selected genes confirmed the accuracy of RNA sequencing results. Bioinformatic analysis of the differentially expressed genes indicated that ferrous iron and cobalt have similar effects on the cellular processes of . Ferrous iron treatment resulted in down-regulation of several oxidative stress tolerance-related genes and up-regulation of the genes in an amino acid ABC transporter operon. Expression of several genes in the arginine deiminase system was down-regulated after ferrous iron and cobalt treatment. Collectively, our results suggested that alters the expression of multiple genes to respond to ferrous iron and cobalt toxicity.
是一种人畜共患病病原体，可导致猪和人类的严重感染。尽管金属是生命所必需的，但过量的金属对细菌是有毒的。除锌以外，目前尚无其他金属可用于研究 对金属毒性的抗性的转录组水平数据。在此，我们通过 RNA 测序探索了 对亚铁和钴毒性的转录组水平变化。过量的亚铁和钴存在时，许多基因的表达水平存在差异。大多数对钴毒性有反应的基因与对亚铁毒性有反应的基因表现出相同的表达趋势。对所选基因的 qRT-PCR 分析证实了 RNA 测序结果的准确性。差异表达基因的生物信息学分析表明，亚铁和钴对 的细胞过程有相似的影响。亚铁处理导致几种与氧化应激耐受相关的基因下调，以及氨基酸 ABC 转运蛋白操纵子中的基因上调。亚铁和钴处理后，精氨酸脱亚氨酶系统中的几个基因的表达下调。总的来说，我们的结果表明， 改变了多个基因的表达，以应对亚铁和钴毒性。
