Fu Mei Ling, Gu Chun Tao
College of Life Sciences, Northeast Agricultural University, Harbin 150030, PR China.
Int J Syst Evol Microbiol. 2019 Sep;69(9):2807-2814. doi: 10.1099/ijsem.0.003563.
A Gram-stain-positive bacterial strain, 395-6.2, was isolated from traditional pickle in Heilongjiang Province, PR China. The bacterium was characterised by a polyphasic approach, including 16S rRNA gene sequence analysis, gene sequence analysis, gene sequence analysis, fatty acid methyl ester (FAME) analysis, determination of DNA G+C content, average nucleotide identity (ANI) analysis, DNA-DNA hybridisation (DDH) and an analysis of phenotypic features. Analysis of the 16S rRNA gene sequence showed that strain 395-6.2 was phylogenetically related to , , , , , , , , , , , , , and . Strain 395-6.2 exhibited 95.7-99.4 % 16S rRNA gene sequence similarities, 85.0-94.0 % gene sequence similarities, 94.2-98.0 % gene sequence similarities to type strains of phylogenetically related species. ANI and DDH values between strain 395-6.2 and type strains of phylogenetically related species were 77.9-87.1 % and 22.5-33.5 %, respectively. Based upon the data obtained in the present study, a novel species, sp. nov., is proposed and the type strain is 395-6.2 (=CCM 8927=NCIMB 15188=LMG 31179).
从中国黑龙江省的传统泡菜中分离出一株革兰氏阳性细菌菌株395-6.2。采用多相分类法对该细菌进行了鉴定，包括16S rRNA基因序列分析、基因序列分析、基因序列分析、脂肪酸甲酯（FAME）分析、DNA G+C含量测定、平均核苷酸同一性（ANI）分析、DNA-DNA杂交（DDH）以及表型特征分析。16S rRNA基因序列分析表明，菌株395-6.2在系统发育上与、、、、、、、、、、、、、相关。菌株395-6.2与系统发育相关物种的模式菌株的16S rRNA基因序列相似性为95.7-99.4%，基因序列相似性为85.0-94.0%，基因序列相似性为94.2-98.0%。菌株395-6.2与系统发育相关物种的模式菌株之间的ANI和DDH值分别为77.9-87.1%和22.5-33.5%。根据本研究获得的数据，提出了一个新物种，sp. nov.，模式菌株为395-6.2（=CCM 8927=NCIMB 15188=LMG 31179）。
