College of Life Sciences, Northeast Agricultural University, Harbin 150030, PR China.
Int J Syst Evol Microbiol. 2019 Oct;69(10):3237-3247. doi: 10.1099/ijsem.0.003619.
Seven novel lactic acid bacterial strains, isolated from traditional Chinese pickle, were characterized using a polyphasic approach, including 16S rRNA gene sequence analysis, gene sequence analysis, gene sequence analysis, determination of DNA G+C content, determination of average nucleotide identity (ANI), DNA-DNA hybridization (DDH), fatty acid methyl ester (FAME) analysis and an analysis of phenotypic features. Strains 382-1, 116-1A, 381-7, 203-3, 218-3 and 398-2 were phylogenetically related to the type strains of subsp. , subsp. , , , , , , , and , having 97.1-99.9 % 16S rRNA gene sequence similarities, less than 89.9 % gene sequence similarities, less than 98.0 % gene sequence similarities, less than 91.2 % ANI values and less than 43.3 % DDH values. Strain 778-3 was phylogenetically related to the type strains of , , , , and , exhibiting 97.0-99.4 % 16S rRNA gene sequence similarities, 78.2-82.1 % gene sequence similarities, 80.0-91.5 % gene sequence similarities, less than 78.6 % ANI values and less than 22.9 % DDH values. Based upon the data of polyphasic characterization obtained in the present study, seven novel species, sp. nov., sp. nov., sp. nov., sp. nov., sp. nov., sp. nov. and sp. nov., are proposed and the type strains are 382-1 (=NCIMB 15187=CCM 8935=LMG 31176), 116-1A (=NCIMB 15181=CCM 8934=LMG 31171), 381-7 (=NCIMB 15186=CCM 8930), 203-3 (=NCIMB 15183=CCM 8933=LMG 31172), 218-3 (=NCIMB 15184=CCM 8932=LMG 31173), 398-2 (=NCIMB 15189=CCM 8931=LMG 31174) and 778-3 (=NCIMB 15191=CCM 8929=LMG 31177), respectively.
从中国传统泡菜中分离得到的 7 株新型乳酸菌菌株，采用多相分类法进行了鉴定，包括 16S rRNA 基因序列分析、基因序列分析、基因序列分析、DNA G+C 含量测定、平均核苷酸同一性 (ANI) 测定、DNA-DNA 杂交 (DDH)、脂肪酸甲酯 (FAME) 分析和表型特征分析。菌株 382-1、116-1A、381-7、203-3、218-3 和 398-2 与乳杆菌亚种、亚种、亚种、亚种、亚种、亚种、亚种、亚种和亚种的模式菌株亲缘关系较近，16S rRNA 基因序列相似度为 97.1-99.9%，基因序列相似度为 89.9%，基因序列相似度为 80.0-91.5%，ANI 值小于 91.2%，DDH 值小于 43.3%。菌株 778-3 与乳杆菌亚种、亚种、亚种、亚种、亚种和亚种的模式菌株亲缘关系较近，16S rRNA 基因序列相似度为 97.0-99.4%，基因序列相似度为 78.2-82.1%，基因序列相似度为 78.6%，ANI 值小于 22.9%，DDH 值小于 22.9%。基于本研究多相分类特征数据，提出了 7 个新种，即 sp. nov.、 sp. nov.、 sp. nov.、 sp. nov.、 sp. nov.、 sp. nov.和 sp. nov.，其模式菌株分别为 382-1（=NCIMB 15187=CCM 8935=LMG 31176）、116-1A（=NCIMB 15181=CCM 8934=LMG 31171）、381-7（=NCIMB 15186=CCM 8930）、203-3（=NCIMB 15183=CCM 8933=LMG 31172）、218-3（=NCIMB 15184=CCM 8932=LMG 31173）、398-2（=NCIMB 15189=CCM 8931=LMG 31174）和 778-3（=NCIMB 15191=CCM 8929=LMG 31177）。
