Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin 150030, PR China.
Int J Syst Evol Microbiol. 2013 Nov;63(Pt 11):4094-4099. doi: 10.1099/ijs.0.053355-0. Epub 2013 May 31.
A Gram-stain-positive bacterial strain, S4-3(T), was isolated from traditional pickle in Heilongjiang Province, China. The bacterium was characterized by a polyphasic approach, including 16S rRNA gene sequence analysis, pheS gene sequence analysis, rpoA gene sequence analysis, dnaK gene sequence analysis, fatty acid methyl ester (FAME) analysis, determination of DNA G+C content, DNA-DNA hybridization and an analysis of phenotypic features. Strain S4-3(T) showed 97.9-98.7 % 16S rRNA gene sequence similarities, 84.4-94.1 % pheS gene sequence similarities and 94.4-96.9 % rpoA gene sequence similarities to the type strains of Lactobacillus nantensis, Lactobacillus mindensis, Lactobacillus crustorum, Lactobacillus futsaii, Lactobacillus farciminis and Lactobacillus kimchiensis. dnaK gene sequence similarities between S4-3(T) and Lactobacillus nantensis LMG 23510(T), Lactobacillus mindensis LMG 21932(T), Lactobacillus crustorum LMG 23699(T), Lactobacillus futsaii JCM 17355(T) and Lactobacillus farciminis LMG 9200(T) were 95.4, 91.5, 90.4, 91.7 and 93.1 %, respectively. Based upon the data obtained in the present study, a novel species, Lactobacillus heilongjiangensis sp. nov., is proposed and the type strain is S4-3(T) ( = LMG 26166(T) = NCIMB 14701(T)).
一株革兰氏阳性细菌菌株 S4-3(T) 从中国黑龙江省的传统泡菜中分离出来。该细菌通过多相分析进行了表征，包括 16S rRNA 基因序列分析、pheS 基因序列分析、rpoA 基因序列分析、dnaK 基因序列分析、脂肪酸甲酯 (FAME) 分析、DNA G+C 含量测定、DNA-DNA 杂交和表型特征分析。菌株 S4-3(T) 与 Lactobacillus nantensis、Lactobacillus mindensis、Lactobacillus crustorum、Lactobacillus futsaii、Lactobacillus farciminis 和 Lactobacillus kimchiensis 的 16S rRNA 基因序列相似性为 97.9-98.7%，pheS 基因序列相似性为 84.4-94.1%，rpoA 基因序列相似性为 94.4-96.9%。S4-3(T) 与 Lactobacillus nantensis LMG 23510(T)、Lactobacillus mindensis LMG 21932(T)、Lactobacillus crustorum LMG 23699(T)、Lactobacillus futsaii JCM 17355(T) 和 Lactobacillus farciminis LMG 9200(T) 的 dnaK 基因序列相似性分别为 95.4%、91.5%、90.4%、91.7%和 93.1%。基于本研究获得的数据，提出了一种新的物种 Lactobacillus heilongjiangensis sp. nov.，其模式菌株为 S4-3(T)（=LMG 26166(T)=NCIMB 14701(T)）。
