Department of Translational Research and Cellular Therapeutics, Diabetes and Metabolism Research Institute, Beckman Research Institute, City of Hope, Duarte, USA.
Sci Rep. 2019 Jun 26;9(1):9295. doi: 10.1038/s41598-019-45678-y.
We developed an optimized Dipheylthiocarbazone or Dithizone (DTZ) with improved physical and chemical properties to characterize human islets and insulin-producing cells differentiated from embryonic stem cells. Application of the newly formulated iDTZ (i stands for islet) over a range of temperatures, time intervals and cell and tissue types found it to be robust for identifying these cells. Through high transition zinc binding, the iDTZ compound concentrated in insulin-producing cells and proved effective at delineating zinc levels in vitro.
我们开发了一种优化的二苯基硫代卡巴腙（Dipheylthiocarbazone 或 Dithizone，DTZ），具有改进的物理和化学性质，用于鉴定人胰岛和从胚胎干细胞分化而来的胰岛素分泌细胞。新配方的 iDTZ（i 代表胰岛）在一系列温度、时间间隔以及细胞和组织类型下的应用表明，它对于鉴定这些细胞具有很强的稳定性。通过与锌的高亲和力结合，iDTZ 化合物在胰岛素分泌细胞中浓缩，并在体外有效地描绘了锌水平。
