Arav Amir, Natan Yehudit
Fertilesafe Ltd., Nes-Ziona, Israel.
Transfus Med Hemother. 2019 Jun;46(3):182-187. doi: 10.1159/000497749. Epub 2019 Mar 25.
Currently vitrification is the method of choice for low-temperature preservation of oocytes and embryos. However, that was not the case until about 10 years ago when freezing methods were used relatively successfully for embryos and investigated (unsuccessfully) for oocyte preservation. In this paper we will review the history of oocyte and embryo cryopreservation and look into ways and methods for overcoming and improving the vitrification method since it suffers from inherent disadvantages since it is a cumbersome, time-consuming and costly procedure.
目前，玻璃化是卵母细胞和胚胎低温保存的首选方法。然而，直到大约10年前情况并非如此，当时冷冻方法相对成功地用于胚胎，并（未成功地）用于卵母细胞保存的研究。在本文中，我们将回顾卵母细胞和胚胎冷冻保存的历史，并探讨克服和改进玻璃化方法的途径和方法，因为它存在固有的缺点，是一个繁琐、耗时且成本高昂的过程。
