Cherbuy Claire, Bellet Déborah, Robert Véronique, Mayeur Camille, Schwiertz Andreas, Langella Philippe
Micalis Institute, INRA, AgroParisTech, Université Paris-Saclay, Jouy-en-Josas, France.
MVZ Institut für Mikroökologie GmbH, Herborn, Germany.
Front Microbiol. 2019 Jun 6;10:1234. doi: 10.3389/fmicb.2019.01234. eCollection 2019.
Alterations in the gut microbiota have been associated with a wide range of pathologies and conditions. Maintaining a well-balanced microbiota is a key factor in sustaining good health. Our aim was to investigate the impact of a resistant starch-containing dietary supplement (SymbioIntest) on the composition of the human gut microbiota and on intestinal short chain fatty acid (SCFA) concentration. Human microbiota-associated mice were used. Ex-germ-free mice were inoculated with fecal suspensions from four different donors. Three weeks later, the mice were orally gavaged for 1 month with either a daily dose of 10 mg of SymbioIntest or the vehicle (water) for the negative control group. The composition of the microbiota and SCFA levels were analyzed by 16S rRNA gene sequencing and gas chromatography, respectively. In three groups of mice, SymbioIntest supplementation increased the concentration of caecal butyrate. This was in conjunction with a remodeling of the gut microbiota. OTUs belonging to the , , and families were affected. In two groups of mice the greatest changes in OTUs were seen in the genus. The supplementation's highest impact was observed in mice inoculated with gut microbiota containing a lower number of and and a higher number of . SymbioIntest supplementation elicited a beneficial effect on the healthy adult gut microbiota by increasing caecal butyrate production and health-promoting taxa. We highlight the fact that screening the gut microbiota may be used for predicting individualized responses to dietary interventions and thus developing personalized nutritional strategies.
肠道微生物群的改变与多种病理状况相关。维持微生物群的平衡是保持健康的关键因素。我们的目的是研究含抗性淀粉的膳食补充剂（SymbioIntest）对人体肠道微生物群组成和肠道短链脂肪酸（SCFA）浓度的影响。使用了与人类微生物群相关的小鼠。将无菌小鼠接种来自四个不同供体的粪便悬液。三周后，给小鼠口服灌胃1个月，一组每日剂量为10 mg的SymbioIntest，另一组为阴性对照组的赋形剂（水）。分别通过16S rRNA基因测序和气相色谱分析微生物群的组成和SCFA水平。在三组小鼠中，补充SymbioIntest增加了盲肠丁酸盐的浓度。这与肠道微生物群的重塑有关。属于 、 、 和 科的操作分类单元（OTU）受到影响。在两组小鼠中，OTU的最大变化出现在 属中。在接种含有较低数量 和 以及较高数量 的肠道微生物群的小鼠中观察到补充剂的最大影响。补充SymbioIntest通过增加盲肠丁酸盐的产生和促进健康的分类群，对健康成年肠道微生物群产生有益影响。我们强调这样一个事实，即筛查肠道微生物群可用于预测对饮食干预的个体化反应，从而制定个性化的营养策略。
