Zhang Wenyi, Guo Yahui, Cheng Yuliang, Zhao Wenjin, Zheng Yuenan, Qian He
State Key Laboratory of Food Science and Technology, Jiangnan University 1800 Lihu Avenue Wuxi 214122 P. R. China
School of Food Science and Technology, Jiangnan University 1800 Lihu Avenue Wuxi 214122 P. R. China.
RSC Adv. 2020 Jun 9;10(37):22164-22175. doi: 10.1039/d0ra01581d. eCollection 2020 Jun 8.
polysaccharides have recently attracted considerable attention due to their excellent bioactivities. However, their extraction procedure is often tedious, time-consuming, and environmentally unfriendly; it even causes damage to their structures and reduces their bioactivities, all of which hinder their further development to some extent. Therefore, the ultrasonic-assisted enzymatic extraction (UAEE) technology was optimized to extract polysaccharides from (SCP) by the response surface methodology. The yields, physicochemical properties, and antioxidant activities of SCPs obtained from UAEE and conventional hot water extraction (HWE) were evaluated. According to the optimal parameters, the yield of SCPs extracted by UAEE reached up to 14.63%, which increased by 68.54% compared with that obtained from the conventional hot water extraction (HWE) method. Additionally, the UAEE methods affected the contents of the polysaccharides, molecular weights, and the molar percentage of the constituent monosaccharides of SCPs. SEM analysis indicated that the microstructures of the two SCPs were notably different. Antioxidant assays showed that both SCPs possessed good antioxidant activities against DPPH, ABTS˙, and hydroxyl radicals . Additionally, the SCPs extracted by UAEE attenuated the HT22 cell neurotoxicity induced by HO by the means of ameliorating cell viability, reducing extracellular LDH release, and decreasing the levels of intracellular ROS. These results provide scientific basis for the further investigation of SCPs as potential neuroprotective agents.
由于多糖具有优异的生物活性，近年来它们受到了广泛关注。然而，其提取过程往往繁琐、耗时且对环境不友好；甚至会对其结构造成破坏并降低其生物活性，所有这些在一定程度上阻碍了它们的进一步发展。因此，采用响应面法对超声辅助酶提取（UAEE）技术进行了优化，以从[具体物质]中提取多糖。对通过UAEE和传统热水提取（HWE）获得的多糖的产率、理化性质和抗氧化活性进行了评估。根据最佳参数，UAEE提取的多糖产率高达14.63%，与传统热水提取（HWE）方法相比提高了68.54%。此外，UAEE方法影响了多糖的含量、分子量以及多糖组成单糖的摩尔百分比。扫描电子显微镜（SEM）分析表明，两种多糖的微观结构明显不同。抗氧化试验表明，两种多糖对二苯基苦味酰基自由基（DPPH）、阳离子自由基（ABTS˙）和羟基自由基均具有良好的抗氧化活性。此外，通过UAEE提取的多糖通过改善细胞活力、减少细胞外乳酸脱氢酶（LDH）释放以及降低细胞内活性氧（ROS）水平，减轻了过氧化氢（HO）诱导的HT22细胞神经毒性。这些结果为进一步研究多糖作为潜在的神经保护剂提供了科学依据。
