Kim Young-Ok, Park Sooyeon, Park In-Suk, Nam Bo-Hye, Kim Dong-Gyun, Yoon Jung-Hoon
Biotechnology Research Division, National Institute of Fisheries Science (NIFS), Gijang, Busan 46083, Republic of Korea.
Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon 16419, Republic of Korea.
Int J Syst Evol Microbiol. 2019 Sep;69(9):2781-2786. doi: 10.1099/ijsem.0.003558.
A Gram-stain-negative, aerobic, non-motile and ovoid or rod-shaped bacterial strain, MRS2, was isolated from an intestine of Nile tilapia () collected from the Republic of Korea. Strain MRS2 grew optimally at 30 °C and in the presence of 0-2.0 % (w/v) NaCl. A neighbour-joining phylogenetic tree of 16S rRNA gene sequences showed that strain MRS2 clustered with the type strains of species. It exhibited the highest 16S rRNA gene sequence similarity (98.5 %) to the type strain of and sequence similarities of 97.4-97.6 % to the type strains of two other species. Strain MRS2 contained MK-6 as the predominant ubiquinone and summed feature 3 (Cω7 and/or Cω6), iso-C 3-OH and iso-C as the major fatty acids. The major polar lipids of strain MRS2 were phosphatidylethanolamine, one unidentified lipid and one unidentified aminolipid. The DNA G+C contents of strain MRS2 were 32.2 mol% or 30.65 mol%. Strain MRS2 exhibited DNA-DNA relatedness values of 12-20 % to the type strains of , and . The average nucleotide identity values between strain MRS2 and five strains of and were 84.8-91.0 %. The phylogenetic, genetic and differential phenotypic properties indicated that strain MRS2 is separated from species. On the basis of the data presented here, strain MRS2 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is MRS2 (=KCTC 62904=NBRC 113550).
从韩国采集的尼罗罗非鱼（Oreochromis niloticus）肠道中分离出一株革兰氏阴性、需氧、不运动且呈卵形或杆状的细菌菌株MRS2。菌株MRS2在30°C以及0 - 2.0%（w/v）NaCl存在的条件下生长最佳。16S rRNA基因序列的邻接法系统发育树表明，菌株MRS2与[具体菌属]物种的模式菌株聚类在一起。它与[具体菌属]模式菌株的16S rRNA基因序列相似性最高（98.5%），与另外两个[具体菌属]物种模式菌株的序列相似性为97.4 - 97.6%。菌株MRS2以MK - 6作为主要泛醌，以特征性总和3（Cω7和/或Cω6）、异C3 - OH和异C作为主要脂肪酸。菌株MRS2的主要极性脂质为磷脂酰乙醇胺、一种未鉴定的脂质和一种未鉴定的氨基脂质。菌株MRS2的DNA G + C含量为32.2 mol%或30.65 mol%。菌株MRS2与[具体菌属]、[具体菌属]和[具体菌属]模式菌株的DNA - DNA相关性值为12 - 20%。菌株MRS2与[具体菌属]和[具体菌属]的五个菌株之间的平均核苷酸同一性值为84.8 - 91.0%。系统发育、遗传和差异表型特征表明，菌株MRS2与[具体菌属]物种不同。基于此处提供的数据，菌株MRS2被认为代表[具体菌属]属的一个新物种，为此提出新物种名称[具体新物种名] sp. nov.。模式菌株为MRS2（=KCTC 62904=NBRC 113550）。
