Kim Hyun Chul, Kim Young-Ok, Park Sooyeon, Nam Bo-Hye, Kim Dong-Gyun, Park Ji-Min, Yoon Jung-Hoon
Genetics and Breeding Research Center, National Institute of Fisheries Science (NIFS), Geoje 53334, Republic of Korea.
Biotechnology Research Division, National Institute of Fisheries Science (NIFS), Gijang, Busan 46083, Republic of Korea.
Int J Syst Evol Microbiol. 2018 Apr;68(4):1233-1237. doi: 10.1099/ijsem.0.002655. Epub 2018 Feb 26.
A Gram-stain-negative, non-motile, aerobic and rod-shaped or ovoid bacterial strain, designated MYP2-2, was isolated from the intestinal tract of a squid (Todarodes pacificus) collected from the East Sea, South Korea, and subjected to a polyphasic taxonomic study. Strain MYP2-2 grew optimally at 30-35 °C and in the presence of 2.0 % (w/v) NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences revealed that strain MYP2-2 belonged to the genus Vitellibacter. Strain MYP2-2 exhibited 16S rRNA gene sequence similarities of 95.4-96.6 % to the type strains of Vitellibacter species and of less than 94.5 % to the type strains of other recognized species examined. Strain MYP2-2 contained menaquinone MK-6 as the predominant respiratory quinone and iso-C15 : 0 and iso-C17 : 0 3-OH as the major fatty acids. The major polar lipids detected in strain MYP2-2 were phosphatidylethanolamine and one unidentified lipid. The DNA G+C content of strain MYP2-2 was 41.6 mol%. Differential phenotypic properties, together with its phylogenetic distinctiveness, revealed that strain MYP2-2 is separated from recognized species of the genus Vitellibacter. On the basis of the data presented, strain MYP2-2 is considered to represent a novel species of the genus Vitellibacter, for which the name Vitellibacter todarodis sp. nov. is proposed. The type strain is MYP2-2 (=KCTC 62141=NBRC 113025).
从韩国东海采集的太平洋褶柔鱼（Todarodes pacificus）肠道中分离出一株革兰氏阴性、无运动性、需氧的杆状或卵形细菌菌株，命名为MYP2-2，并对其进行了多相分类研究。菌株MYP2-2在30-35 °C和2.0%（w/v）NaCl存在的条件下生长最佳。基于16S rRNA基因序列构建的邻接系统发育树表明，菌株MYP2-2属于黄杆菌属（Vitellibacter）。菌株MYP2-2与黄杆菌属各模式菌株的16S rRNA基因序列相似性为95.4-96.6%，与其他已确认物种的模式菌株的相似性低于94.5%。菌株MYP2-2以甲基萘醌MK-6作为主要呼吸醌，以异-C15 : 0和异-C17 : 0 3-OH作为主要脂肪酸。在菌株MYP2-2中检测到的主要极性脂类为磷脂酰乙醇胺和一种未鉴定的脂类。菌株MYP2-2的DNA G+C含量为41.6 mol%。不同的表型特征及其系统发育独特性表明，菌株MYP2-2与黄杆菌属已确认的物种不同。根据所提供的数据，菌株MYP2-2被认为代表黄杆菌属的一个新物种，为此提出了黄杆菌太平洋褶柔鱼（Vitellibacter todarodis sp. nov.）这一名称。模式菌株为MYP2-2（=KCTC 62141=NBRC 113025）。
