Heo Jun, Hamada Moriyuki, Cho Hayoung, Weon Hang-Yeon, Kim Jeong-Seon, Hong Seong-Beom, Kim Soo-Jin, Kwon Soon-Wo
Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration, Wanju-gun, Jeollabuk-do 55365, Republic of Korea.
NITE Biological Resource Center (NBRC), National Institute of Technology and Evaluation, 2-5-8 Kazusakamatari, Kisarazu, Chiba 292-0818, Japan.
Int J Syst Evol Microbiol. 2019 Sep;69(9):2801-2806. doi: 10.1099/ijsem.0.003564.
A taxonomic study of a Gram-stain-positive, rod-shaped, non-motile, non-spore-forming, catalase-negative bacterium, isolated from the gut of an insect, collected from the mountainous area of Seoraksan, Yangyang-gun, Republic of Korea, was conducted. Its 16S rRNA gene sequence showed high similarity values to LMG 24286 (95.9 %), 2L24P13 (95.9 %), M75 (95.7 %) and 257 (95.7 %). The phylogenetic tree indicated that the novel organism formed a cluster with LMG 24286, 2L24P13, M75 and 257. The G+C content was 41.1 mol% on the basis of the whole-genome sequence. Polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, two unidentified aminophospholipids, one unidentified phospholipid and four unidentified lipids. The major cellular fatty acids were C 9, C, C, summed feature 8 (Cω7 and/or Cω6) and summed feature 8 (C 7 and/or C 6). The cell-wall peptidoglycan was of A4 type with the interpeptide bridge of Gly-d-Glu. Based on these results, strain 26KH-42 could be classified as a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 26KH-42 (=KACC 18423=NBRC 113066).
对从韩国江原道襄阳郡雪岳山山区采集的昆虫肠道中分离出的一株革兰氏染色阳性、杆状、不运动、不产芽孢、过氧化氢酶阴性细菌进行了分类学研究。其16S rRNA基因序列与LMG 24286（95.9%）、2L24P13（95.9%）、M75（95.7%）和257（95.7%）显示出高相似性值。系统发育树表明，该新生物体与LMG 24286、2L24P13、M75和257形成一个聚类。基于全基因组序列，G+C含量为41.1mol%。极性脂质由双磷脂酰甘油、磷脂酰甘油、两种未鉴定的氨基磷脂、一种未鉴定的磷脂和四种未鉴定的脂质组成。主要细胞脂肪酸为C9、C、C、总和特征8（Cω7和/或Cω6）和总和特征8（C7和/或C6）。细胞壁肽聚糖为A4型，肽间桥为Gly-d-Glu。基于这些结果，菌株26KH-42可被分类为该属的一个新物种，为此提出新物种名称sp. nov.。模式菌株为26KH-42（=KACC 18423=NBRC 113066）。
