Department of Chemistry and Biochemistry , University of Oklahoma , Norman , Oklahoma 73019 , United States.
Anal Chem. 2019 Jul 16;91(14):9018-9024. doi: 10.1021/acs.analchem.9b01311. Epub 2019 Jun 27.
Analyzing cellular constituents on the single-cell level through mass spectrometry (MS) allows for a wide range of compounds to be studied simultaneously. However, there is a need for quantitative single-cell mass spectrometry (qSCMS) methods to fully characterize drug efficacy from individual cells within cell populations. In this study, qSCMS experiments were carried out using the Single-probe MS technique. The method was successfully used to perform rapid absolute quantifications of the anticancer drug irinotecan in individual mammalian cancer cells under ambient conditions in real time. Traditional liquid chromatography/mass spectrometry (LC/MS) quantifications of irinotecan in cell lysate samples were used to compare the results from Single-probe qSCMS. This technique showcases heterogeneity of drug efficacy on the single-cell level.
通过质谱分析（MS）在单细胞水平上分析细胞成分，可以同时研究多种化合物。然而，需要定量单细胞质谱分析（qSCMS）方法来全面描述细胞群体中单个细胞的药物疗效。在这项研究中，使用单探针 MS 技术进行 qSCMS 实验。该方法成功地用于实时在环境条件下对单个哺乳动物癌细胞中的抗癌药物伊立替康进行快速绝对定量。传统的细胞裂解物样品中的伊立替康的液相色谱/质谱（LC/MS）定量分析用于比较单探针 qSCMS 的结果。该技术展示了单细胞水平上药物疗效的异质性。
