成簇微小RNA hsa-miR-221-3p/hsa-miR-222-3p及其靶向基因可能是肝细胞癌的预后预测指标。
Clustered microRNAs hsa-miR-221-3p/hsa-miR-222-3p and their targeted genes might be prognostic predictors for hepatocellular carcinoma.
作者信息
Wang Xiangkun, Liao Xiwen, Huang Ketuan, Zeng Xianmin, Liu Zhengqian, Zhou Xin, Yu Tingdong, Yang Chengkun, Yu Long, Wang Qiaoqi, Han Chuangye, Zhu Guangzhi, Ye Xinping, Peng Tao
机构信息
Department of Hepatobiliary Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, Guangxi Province, China.
Department of Hepatobiliary and Pancreatic Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450000, Henan Province, China.
出版信息
J Cancer. 2019 Jun 2;10(11):2520-2533. doi: 10.7150/jca.29207. eCollection 2019.
: MicroRNAs (miRNAs) have been explored in malignancies. We investigated the functions of clustered miRNAs hsa-miR-221/222-3p in hepatocellular carcinoma (HCC). : Human miRNA tissue atlas website was determined expression levels in liver tissue. Four databases, TarBase, miRTarBase, miRecords and miRPathDB, were found experimentally validated target genes of clustered miRNAs. TargetScanHuman was predicted target genes. The STRING website was depicted protein-protein interaction (PPI) networks. The OncoLnc website analyzed prognostic values for hsa-miR-221/222-3p and their target genes. The MCODE plugin calculated modules of PPI networks. Receiver operating characteristic (ROC) curves were predicted 1, 3, and 5 years prognostic values. : Expression of clustered miRNAs was high in liver tissues. A total of 1577 target genes were identified. Enrichment analysis showed that target genes were enriched mainly in cancer, Wnt signaling and ErbB signaling pathways. Two modules were calculated using PPI networks. Has-miR-221-3p was not associated with prognosis ( = 0.401). Has-miR-222-3p and target genes , , , , and of the clustered miRNAs were associated with HCC survival (all < 0.05). Has-miR-222-3p, , and showed good performance of ROC in prognosis prediction at 1, 3, and 5 years (all area under curves > 0.600). : Has-miR-222-3p and target genes especially , , may serve as prognostic predictors for HCC.
: 微小RNA（miRNA）已在恶性肿瘤中得到研究。我们研究了成簇miRNA hsa-miR-221/222-3p在肝细胞癌（HCC）中的功能。: 通过人类miRNA组织图谱网站确定肝脏组织中的表达水平。在四个数据库TarBase、miRTarBase、miRecords和miRPathDB中查找成簇miRNA的实验验证靶基因。使用TargetScanHuman预测靶基因。通过STRING网站描绘蛋白质-蛋白质相互作用（PPI）网络。利用OncoLnc网站分析hsa-miR-221/222-3p及其靶基因的预后价值。使用MCODE插件计算PPI网络的模块。绘制受试者工作特征（ROC）曲线以预测1年、3年和5年的预后价值。: 成簇miRNA在肝脏组织中表达较高。共鉴定出1577个靶基因。富集分析表明，靶基因主要富集于癌症、Wnt信号通路和ErbB信号通路。使用PPI网络计算出两个模块。Has-miR-221-3p与预后无关（ = 0.401）。Has-miR-222-3p以及成簇miRNA的靶基因 、 、 、 、 和 与HCC生存相关（均 < 0.05）。Has-miR-222-3p、 、 和 在1年、3年和5年的预后预测中表现出良好的ROC性能（曲线下面积均> 0.600）。: Has-miR-222-3p及其靶基因尤其是 、 可能作为HCC的预后预测指标。
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