Department of Urology, the Fifth Medical Center, Chinese PLA General Hospital, Beijing, China.
College of Life Science, Hebei University, Hebei, China.
Int Braz J Urol. 2019 Sep-Oct;45(5):916-924. doi: 10.1590/S1677-5538.IBJU.2018.0535.
This study aims to investigate the association of filamin A with the function and morphology of prostate cancer (PCa) cells, and explore the role of filamin A in the development of PCa, in order to analyze its significance in the evolvement of PCa.
A stably transfected cell line, in which filamin A expression was suppressed by RNA interference, was first established. Then, the effects of the suppression of filamin A gene expression on the biological characteristics of human PCa LNCaP cells were observed through cell morphology, in vitro cell growth curve, soft agar cloning assay, and scratch test.
A cell line model with a low expression of filamin A was successfully constructed on the basis of LNCaP cells. The morphology of cells transfected with plasmid pSilencer-filamin A was the following: Cells were loosely arranged, had less connection with each other, had fewer tentacles, and presented a fibrous look. The growth rate of LNCap cells was faster than cells transfected with plasmid pSilencer-filamin A (P<0.05). The clones of LNCap cells in the soft agar cloning assay was significantly fewer than that of cells stably transfected with plasmid pSilencer-filamin A (P<0.05). Cells stably transfected with plasmid pSilencer-filamin A presented with a stronger healing and migration ability compared to LNCap cells (healing rate was 32.2% and 12.1%, respectively; P<0.05).
The expression of the filamin A gene inhibited the malignant development of LNCap cells. Therefore, the filamin A gene may be a tumor suppressor gene.
本研究旨在探讨细丝蛋白 A 与前列腺癌（PCa）细胞功能和形态的关系，并探讨细丝蛋白 A 在 PCa 发生发展中的作用，分析其在 PCa 演变中的意义。
首先建立了通过 RNA 干扰抑制细丝蛋白 A 表达的稳定转染细胞系，然后通过细胞形态、体外细胞生长曲线、软琼脂克隆试验和划痕试验观察抑制细丝蛋白 A 基因表达对人前列腺癌细胞 LNCaP 生物学特性的影响。
在 LNCaP 细胞的基础上成功构建了低表达细丝蛋白 A 的细胞系模型。转染质粒 pSilencer-filamin A 的细胞形态为：细胞排列疏松，彼此连接较少，触角较少，呈纤维状。LNCap 细胞的生长速度快于转染质粒 pSilencer-filamin A 的细胞（P<0.05）。软琼脂克隆试验中 LNCap 细胞的克隆明显少于稳定转染质粒 pSilencer-filamin A 的细胞（P<0.05）。与 LNCap 细胞相比，稳定转染质粒 pSilencer-filamin A 的细胞具有更强的愈合和迁移能力（愈合率分别为 32.2%和 12.1%；P<0.05）。
细丝蛋白 A 基因的表达抑制了 LNCap 细胞的恶性发展。因此，细丝蛋白 A 基因可能是一种肿瘤抑制基因。
