Kudo A, Pravtcheva D, Sakaguchi N, Ruddle F H, Melchers F
Basel Institute for Immunology, Basel, Switzerland.
Genomics. 1987 Nov;1(3):277-9. doi: 10.1016/0888-7543(87)90055-3.
The chromosomal location of the murine lambda 5 gene was analyzed by Southern hybridization using restriction enzyme-digested DNA from a panel of 15 mouse X hamster somatic cell hybrids. Sequences homologous with those of lambda 5 DNA were detected in DNA of 5 hybrids. In all 5 hybrids lambda 5 was contained in restriction fragments of equal sizes, the lengths of which indicated that the germline configuration of lambda 5 with three exons and the restriction sites expected from its genomic structure were present. Southern hybridization with the murine lambda 1 gene as a probe detected the same 5 hybrids as positive. The only mouse chromosome present on all of the positive hybrids, and absent from negative ones, was number 16. We conclude that lambda 5 is situated on the same chromosome as lambda 1, i.e., on the murine chromosome 16.
利用来自一组15个小鼠X仓鼠体细胞杂种的经限制性内切酶消化的DNA，通过Southern杂交分析了小鼠λ5基因的染色体定位。在5个杂种的DNA中检测到与λ5 DNA序列同源的序列。在所有5个杂种中，λ5都包含在大小相等的限制性片段中，其长度表明存在具有三个外显子的λ5种系构型以及从其基因组结构预期的限制性位点。以小鼠λ1基因为探针的Southern杂交检测到相同的5个杂种为阳性。所有阳性杂种均存在且阴性杂种均不存在的唯一小鼠染色体是16号染色体。我们得出结论，λ5与λ1位于同一条染色体上，即小鼠16号染色体上。
