Emanuel Institute for Biochemical Physics, Russian Academy of Sciences, Moscow, Russia.
V.L. Talrose Institute for Energy Problems of Chemical Physics, N.N. Semenov Federal Center of Chemical Physic of RAS, Moscow, Russia.
Eur J Mass Spectrom (Chichester). 2020 Apr;26(2):158-161. doi: 10.1177/1469066719860076. Epub 2019 Jul 10.
The study of protein misfolding and post-translational processing abnormalities is a promising diagnostic approach for socially significant pathologies associated with the accumulation of abnormal forms of proteins. Recently, it was shown that amyloid-like aggregates can be observed in the urine of pregnant women with preeclampsia, which is the most severe hypertensive complication that can lead to fateful outcomes. The protein composition of urine aggregates may clarify the molecular mechanisms underlying the pathology and has not yet been studied in detail. Using a proteomic approach based on high-resolution mass spectrometry, we studied the protein composition of amyloid-like structures that aggregate in the presence of Congo red azo-dye in the urine of pregnant women with preeclampsia. Fragments of β-sheets of α-1-antitrypsin, complement 3, haptoglobin, ceruloplasmin, and trypstatin were identified as most likely targets for Congo red binding.
蛋白质错误折叠和翻译后加工异常的研究是一种有前途的诊断方法，可用于研究与异常蛋白质积累相关的具有社会意义的疾病。最近的研究表明，子痫前期孕妇的尿液中可以观察到类淀粉样物聚集，这是最严重的高血压并发症，可能导致致命后果。尿液聚集物的蛋白质组成可能阐明潜在的病理机制，但尚未详细研究。本研究使用基于高分辨率质谱的蛋白质组学方法，研究了在刚果红偶氮染料存在下聚集的子痫前期孕妇尿液中的类淀粉样结构的蛋白质组成。α-1-抗胰蛋白酶、补体 3、触珠蛋白、铜蓝蛋白和胰蛋白酶抑制剂的β-片层片段被鉴定为最有可能与刚果红结合的靶标。
