Department of General Surgery, Affiliated Hospital of Nantong University, Nantong, China.
Research center of clinical medicine, Affiliated Hospital of Nantong University, Nantong, China.
J Mater Sci Mater Med. 2019 Jul 10;30(7):85. doi: 10.1007/s10856-019-6287-x.
Pancreatic transplantation remains the only cure for diabetes, but the shortage of donors limits its clinical application. Whole organ decellularized scaffolds offer a new opportunity for pancreatic organ regeneration; however inadequate endothelialization and vascularization can prevent sufficient transport of oxygen and nutrient supplies to the transplanted organ, as well as leading unwanted thrombotic events. In the present study, we explored the re-endothelialization of rat pancreatic acellular scaffolds via circulation perfusion using human skin fibroblasts (FBs) and human umbilical vein endothelial cells (HUVECs). Our results revealed that the cell adhesion rate when these cells were co-cultured was higher than under control conditions, and this increase was associated with increased release of growth factors including VEGF, FGFb, EGF, and IGF-1 as measured by ELISA. When these recellularized organs were implanted in vivo for 28 days in rat dorsal subcutaneous pockets, we found that de novo vasculature formation in the co-culture samples was superior to the control samples. Together these results suggest that endothelial cell and FB co-culture enhances the re-endothelialization and vascularization of pancreatic acellular scaffolds.
胰腺移植仍然是糖尿病的唯一治愈方法，但供体短缺限制了其临床应用。整个器官去细胞支架为胰腺器官再生提供了新的机会；然而，内皮化和血管化不足会阻止足够的氧气和营养物质输送到移植器官，并导致不必要的血栓形成事件。在本研究中，我们通过循环灌注利用人皮肤成纤维细胞（FB）和人脐静脉内皮细胞（HUVEC）探索了大鼠去细胞胰腺支架的再内皮化。我们的结果表明，当这些细胞共培养时，细胞黏附率高于对照条件，并且这种增加与生长因子的释放增加有关，这些生长因子包括通过 ELISA 测量的 VEGF、FGFb、EGF 和 IGF-1。当这些再细胞化的器官在大鼠背部皮下囊内行 28 天体内植入时，我们发现共培养样品中的新生血管形成优于对照样品。这些结果表明，内皮细胞和成纤维细胞共培养增强了胰腺去细胞支架的再内皮化和血管化。
