Haidukowski Miriam, Casamassima Eliana, Cimmarusti Maria Teresa, Branà Maria Teresa, Longobardi Francesco, Acquafredda Pasquale, Logrieco Antonio, Altomare Claudio
Department of Biology, Agriculture and Food Science, Institute of Sciences of Food Production, National Research Council (CNR), Bari, Italy.
Department of Chemistry, University of Bari, Bari, Italy.
Front Microbiol. 2019 Jun 25;10:1386. doi: 10.3389/fmicb.2019.01386. eCollection 2019.
Aflatoxin B (AfB) is a carcinogenic mycotoxin that contaminates food and feed worldwide. We determined the AfB-adsorption capability of non-viable mycelium, an edible fungus, as a potential means for removal of AfB from contaminated solutions. Lyophilized mycelium was produced and made enzymatically inert by sterilization at high temperatures. The material thus obtained was characterized by scanning electron microscopy with regard to the morpho-structural properties of the mycotoxin-adsorbing surfaces. The active surfaces appeared rough and sponge-like. The AfB-mycelium system reached equilibrium at 37°C, 30 min, and pH 5-7, conditions that are compatible with the gastro-intestinal system of animals. The system remained stable for 48 h at room temperature, at pH 3, pH 7, and pH 7.4. A thermodynamic study of the process showed that this is a spontaneous and physical adsorption process, with a maximum of 85 ± 13% of removal efficiency of AfB by mycelium. These results suggest that biosorbent materials obtained from the mycelium of the mushroom could be used as a low-cost and effective feed additive for AfB detoxification.
黄曲霉毒素B（AfB）是一种致癌霉菌毒素，在全球范围内污染食品和饲料。我们测定了一种可食用真菌——非活性菌丝体对AfB的吸附能力，将其作为从受污染溶液中去除AfB的一种潜在方法。制备了冻干菌丝体，并通过高温灭菌使其酶活性丧失。通过扫描电子显微镜对由此获得的材料进行了表征，以研究其吸附霉菌毒素表面的形态结构特性。活性表面显得粗糙且呈海绵状。AfB-菌丝体系统在37°C、30分钟和pH值5-7的条件下达到平衡，这些条件与动物的胃肠道系统相适应。该系统在室温、pH值3、pH值7和pH值7.4的条件下保持稳定48小时。对该过程的热力学研究表明，这是一个自发的物理吸附过程，菌丝体对AfB的去除效率最高可达85±13%。这些结果表明，从蘑菇菌丝体制备的生物吸附材料可作为一种低成本且有效的饲料添加剂用于AfB解毒。
