ENT Department, Guangzhou Hospital of Integrated Traditional and West Medicine, No. 87 Yingbin Road, Huadu District, Guangzhou 510800, Guangdong Province, China
ENT Department, Guangzhou Hospital of Integrated Traditional and West Medicine, No. 87 Yingbin Road, Huadu District, Guangzhou 510800, Guangdong Province, China.
Biosci Rep. 2019 Jul 29;39(7). doi: 10.1042/BSR20190673. Print 2019 Jul 31.
Honeysuckle has antiviral, antioxidative and anti-inflammatory properties. Allergic rhinitis (AR) is induced by immunoglobulin E (IgE)-mediated inflammatory reaction. Our study investigates whether honeysuckle extract (HE) has therapeutic effect on AR. An AR model of mice was established by ovalbumin (OVA). Hematoxylin-Eosin staining was used to assess nasal mucosa damage. Enzyme-linked immunosorbent assay (ELISA) was performed to determine serum histamine, IgE and interleukin (IL)-2, IL-4, IL-17 and interferon-γ (IFN-γ) from nasal lavage fluid. Western blot was carried out to analyze the protein level from nasal mucosa tissue. We found that HE not only decreased nasal rubbing and sneezing in AR mice, but also reduced AR-induced damage to nasal mucosa. Moreover, HE lowered the levels of serum IgE and histamine and inhibited IL-4 and IL-17 levels from AR mice but raised IL-2 and IFN-γ levels in AR-induced nasal lavage fluid. Our results also showed that HE elevated the protein levels of forkhead box P3 (Foxp3) and T-box transcription factor (T-bet) in AR-induced nasal mucosa tissue, whereas it inhibited signal transducer and activator of transcription (STAT) 3 and GATA binding protein 3 (GATA-3) protein levels. By regulating AR-induced inflammatory reaction and autoimmune response, HE also relieved OVA-induced AR. Thus, HE could be used as a potential drug to treat AR.
金银花具有抗病毒、抗氧化和抗炎特性。过敏性鼻炎（AR）是由免疫球蛋白 E（IgE）介导的炎症反应引起的。本研究旨在探讨金银花提取物（HE）对 AR 是否具有治疗作用。通过卵清蛋白（OVA）建立 AR 小鼠模型。通过苏木精-伊红（H&E）染色评估鼻黏膜损伤。通过酶联免疫吸附试验（ELISA）测定鼻洗液中血清组胺、IgE 和白细胞介素（IL）-2、IL-4、IL-17 和干扰素-γ（IFN-γ）的含量。通过 Western blot 分析鼻黏膜组织中的蛋白水平。结果表明，HE 不仅减少了 AR 小鼠的鼻挠和打喷嚏次数，还减轻了 AR 引起的鼻黏膜损伤。此外，HE 降低了 AR 小鼠血清 IgE 和组胺水平，抑制了 AR 诱导的鼻洗液中 IL-4 和 IL-17 水平，同时升高了 AR 诱导的鼻洗液中 IL-2 和 IFN-γ 水平。研究结果还表明，HE 上调了 AR 诱导的鼻黏膜组织中叉头框 P3（Foxp3）和 T 盒转录因子（T-bet）的蛋白水平，同时抑制了信号转导和转录激活因子（STAT）3 和 GATA 结合蛋白 3（GATA-3）的蛋白水平。通过调节 AR 诱导的炎症反应和自身免疫反应，HE 缓解了 OVA 诱导的 AR。因此，HE 可作为治疗 AR 的潜在药物。
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