Zoonosis Research Center, Department of Infection Biology, School of Medicine, Wonkwang University, Iksan 570-749, Korea.
Department of Microbiology, Bach Mai Hospital, Hanoi 100000, Vietnam.
Int J Mol Sci. 2019 Jul 15;20(14):3464. doi: 10.3390/ijms20143464.
Dengue, one of the most prevalent illnesses caused by dengue viruses that are members of the genus , is a significant global health problem. However, similar clinical symptoms and high antigenic homologies with other Flaviviruses in the endemic area pose difficulties for differential diagnosis of dengue from other arbovirus infections. Here, we investigated four types of recombinant envelope protein domain III (DV-rED III) derived from four dengue virus (DENV) serotypes for diagnostic potential in detecting IgM in acute phase (mainly 2-3 days after onset of fever). Each independent DV-1, -3, and -4-rED III-ELISA showed less than 60% sensitivity, but the combined results of DV-1, -3, and -4-rED III-ELISA led to sensitivity of 81.82% (18/22) (95% CI, 59.72 to 94.81) and 100% specificity (46/46) (95% CI, 92.29 to 100.00) as each antigen compensated the other antigen-derived negative result. In conclusion, the independent combination of data derived from each recombinant antigen (DV1-, DV3-, and DV4-rED III) showed comparable efficacy for the detection of IgM in patients with acute-phase dengue infection.
登革热是由登革病毒引起的最常见疾病之一，也是一个全球性的重大健康问题。然而，在流行地区，与其他黄病毒相似的临床症状和高抗原同源性，给登革热与其他虫媒病毒感染的鉴别诊断带来了困难。在这里，我们研究了来自 4 种登革病毒（DENV）血清型的 4 种重组包膜蛋白结构域 III（DV-rED III），以评估其在检测急性期中 IgM 的诊断潜力（主要是发热后 2-3 天）。每种独立的 DV-1、-3 和 -4-rED III-ELISA 的敏感性均低于 60%，但 DV-1、-3 和 -4-rED III-ELISA 的联合结果导致敏感性为 81.82%（18/22）（95%CI，59.72-94.81）和 100%特异性（46/46）（95%CI，92.29-100.00），因为每种抗原都补偿了其他抗原的阴性结果。总之，来自每种重组抗原（DV1-、DV3-和 DV4-rED III）的独立数据组合在检测急性登革热感染患者中的 IgM 方面显示出相当的效果。
