Galarza Janeth I, Arredondo Vega Bertha O, Villón Jimmy, Henríquez Vitalia
Facultad de Ciencias del Mar, Universidad Estatal Península de Santa Elena, Provincia de Santa Elena, Ecuador.
Laboratorio de Biotecnología de Microalgas, Centro de Investigaciones Biológicas del Noroeste (CIBNOR), La Paz, Baja California Sur, Mexico.
Biotechnol Rep (Amst). 2019 Jun 17;23:e00351. doi: 10.1016/j.btre.2019.e00351. eCollection 2019 Sep.
is the richest biological source of astaxanthin under unfavorable growing conditions. Many reports have discussed the optimal astaxanthin extraction methods. Free-astaxanthin could be still hindered by microalgae extracts composition or by prolonged extraction times. In this study we evaluated the effect of enzymolysis and saponification deesterification processes of astaxanthin and its carotenoid precursors under high irradiance and nitrogen deprivation stress time conditions. Results showed that cholesterol esterase facilitated astaxanthin deesterification (975.65 μg mg DW) while saponification positively affected zeaxanthin (1038.68 μg mg DW).
在不利的生长条件下，是虾青素最丰富的生物来源。许多报告都讨论了虾青素的最佳提取方法。游离虾青素仍可能受到微藻提取物成分或延长提取时间的阻碍。在本研究中，我们评估了在高辐照度和氮剥夺胁迫时间条件下，虾青素及其类胡萝卜素前体的酶解和皂化脱酯过程的影响。结果表明，胆固醇酯酶促进了虾青素脱酯（975.65μg mg DW），而皂化对玉米黄质有积极影响（1038.68μg mg DW）。
