Section of Experimental Oncology und Nanomedicine (SEON), ENT-Department, Universitätsklinikum Erlangen, Friedrich-Alexander Universität Erlangen-Nürnberg, 91054 Erlangen, Germany.
Molecules. 2019 Jul 16;24(14):2588. doi: 10.3390/molecules24142588.
Magnetic drug targeting utilizes an external magnetic field to target superparamagnetic iron oxide nanoparticles (SPIONs) and their cargo to the diseased vasculature regions. In the arteries, the flow conditions affect the behavior of magnetic particles and the efficacy of their accumulation. In order to estimate the magnetic capture of SPIONs in more physiological-like settings, we previously established an ex vivo model based on human umbilical cord arteries. The artery model was employed in our present studies in order to analyze the effects of the blood components on the efficacy of magnetic targeting, utilizing 2 types of SPIONs with different physicochemical characteristics. In the presence of freshly isolated human plasma or whole blood, a strong increase in iron content measured by AES was observed for both particle types along the artery wall, in parallel with clotting activation due to endogenous thrombin generation in plasma. Subsequent studies therefore utilized SPION suspensions in serum and washed red blood cells (RBCs) at hematocrit 50%. Interestingly, in contrast to cell culture medium suspensions, magnetic accumulation of circulating SPION-3 under the external magnet was achieved in the presence of RBCs. Taken together, our data shows that the presence of blood components affects, but does not prevent, the magnetic accumulation of circulating SPIONs.
磁靶向药物利用外部磁场将超顺磁性氧化铁纳米粒子（SPION）及其载药靶向到病变血管区域。在动脉中，流动条件会影响磁性粒子的行为及其聚集的效果。为了在更类似生理的环境中评估 SPION 的磁捕获效果，我们之前建立了一种基于人脐带动脉的离体模型。本研究中使用了动脉模型，以分析血液成分对磁靶向效果的影响，使用了两种具有不同理化特性的 SPION。在新鲜分离的人血浆或全血存在的情况下，两种粒子类型在动脉壁上的铁含量均通过 AES 检测到明显增加，这与由于血浆中内源性凝血酶生成导致的凝血激活平行。因此，后续研究在血清和 50%红细胞压积（HCT）的洗涤红细胞（RBC）中使用 SPION 悬浮液。有趣的是，与细胞培养基悬浮液相比，在 RBC 存在的情况下，循环 SPION-3 在外部磁场下的磁性聚集得以实现。总之，我们的数据表明，血液成分的存在会影响，但不会阻止循环 SPION 的磁性聚集。
