Shanghai Key Laboratory of Green Chemistry and Chemical Processes, School of Chemistry and Molecular Engineering, East China Normal University, Dongchuan Road 500, Shanghai, 200241, China.
Angew Chem Int Ed Engl. 2019 Sep 23;58(39):13948-13953. doi: 10.1002/anie.201907210. Epub 2019 Aug 14.
A biosensor was created for the simultaneous monitoring of endogenous H S and H S in mouse brains and exploring their roles in activation of the TRPA1 channel under two types of brain disease models: ischemia and Alzheimer's disease (AD). Based on DFT calculations and electrochemical measurements, two probes, 3,4-bis((2-fluoro-5-nitrobenzoyl)oxy)-benzoic acid (M ) and N-(4-(2,5-dinitrophenoxy) phenyl)-5-(1, 2-dithiolan-3-yl)pentanamide (M ), were synthesized for specific recognition of H S and H S. Through co-assembly of the two probes at the mesoporous gold film with good anti-biofouling ability and electrocatalytic activity, this microsensor showed high selectivity for H S and H S against potential biological interferences. The biosensor can simultaneously determine the concentration of H S from 0.2 to 50 μm, as well as that of H S from 0.2 to 40 μm. The expression of TRPA1 protein positively correlated with levels of H S under both ischemia and AD.
研制了一种用于同时监测内源性 H2S 和 H2S的生物传感器，并在两种脑疾病模型（缺血和阿尔茨海默病（AD））下，探索了它们在激活 TRPA1 通道中的作用。基于 DFT 计算和电化学测量，合成了两种探针，3,4-双（（2-氟-5-硝基苯甲酰）氧基）-苯甲酸（M）和 N-（4-（2,5-二硝基苯氧基）苯基）-5-（1,2-二硫杂戊烷-3-基）戊酰胺（M），用于特异性识别 H2S 和 H2S。通过将两种探针在具有良好抗生物污染能力和电催化活性的介孔金膜中共同组装，该微传感器对 H2S 和 H2S 表现出高选择性，可有效抵抗潜在的生物学干扰。该生物传感器可以同时测定 H2S 的浓度范围为 0.2 到 50μm，以及 H2S 的浓度范围为 0.2 到 40μm。在缺血和 AD 两种情况下，TRPA1 蛋白的表达与 H2S 的水平呈正相关。
